Effects of polyunsaturated fatty acids supplemented in medium on primary culture

• Project/Area Number: 16K12864
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Yamagata University
• Principal Investigator: NAKAMURA TAKAO 山形大学, 大学院医学系研究科, 教授 (00142654)
• Co-Investigator(Renkei-kenkyūsha): KUSUNOKI Masataka 名古屋大学, 総合保健体育科学センター, 教授 (80214956) ; SATO Daisuke 山形大学, 大学院医学系研究科, 助教 (60536960)
• Research Collaborator: FENG Zhonggang ; TAKAHASHI Kazuaki ; SASAKI Hiroyuki ; KOSAKA Kaoru ; IGUCHI Seiya ; SAITO Jo ; TAKAHASHI Shinya ; OTORI Maki ; YANO Mizuna
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 再生医工学 / 脂質 / 初代培養 / 心筋細胞 / 脂肪細胞

Outline of Final Research Achievements

Primary culture of rat cardiomyocytes was conducted, using medium containing one of 5 fatty acids including docosahexaenoic (DHA) and arachidonic (AA) acids. Maximal contractile performance was obtained in cases of 20μM DHA and 50μA AA, with large difference in gene expression. Although combined supplementation of 10μM DHA and 10-20μM AA showed maximal performance of contractility, its level stayed rather close to that obtained in single DHA supplementation.
Primary culture of adult rat adipocytes was superior to that of juvenile one. However, even in the case of DHA supplementation, retaining of adipocytes remained difficult. These results may show effects of fatty acids are dependent on cell species.

Research Products

• [Journal Article] Unknown biological effects of L-glucose, ALA, and PUFA (2017)
  • Author(s): Yamada, K., Sato, D., Nakamura, T., Amano, H., and Morimoto, Y.
  • Journal Title: J. Physiol. Sci. Volume: 67 Issue: 5 Pages: 539-548
  • DOI: 10.1007/s12576-017-0544-x
  • NAID: 40021301945
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Supplementation of arachidonic or docosahexaenoic acid in culture medium improves contractile performance of cardiomyocytes (2018)
  • Author(s): Nakamura T, Sato D, Saito J, Feng Z, Takahashi K, Kusunoki M
  • Organizer: World Cong Med Physics Biomed Eng
  • Int'l Joint Research
• [Presentation] 多価不飽和脂肪酸の培地添加は培養心筋細胞の拍動収縮能を改善する (2018)
  • Author(s): 佐藤大介，大鳥真紀，馮忠剛，楠正隆，中村孝夫
  • Organizer: 第57回日本生体医工学会大会
  • Invited
• [Presentation] ラット初代培養心筋細胞の拍動能に及ぼすドコサヘキサエン酸及びアラキドン酸の効果 (2017)
  • Author(s): 斉藤丈，小坂薫，佐藤大介，馮忠剛，楠正隆，中村孝夫
  • Organizer: 第56回日本生体医工学会大会
• [Presentation] ラット培養心筋細胞の拍動能及び脂肪酸組成に及ぼす多価不飽和脂肪酸の効果 (2017)
  • Author(s): 佐藤大介，小坂薫，斉藤丈，楠正隆，中村孝夫
  • Organizer: 第69回日本生物工学会大会
• [Presentation] Polyunsaturated fatty acids to maintain cardiomyocyte function in culture (2016)
  • Author(s): Nakamura T
  • Organizer: Bio-IST Symp
  • Place of Presentation: Kuala Lumpur (Malaysia)
  • Int'l Joint Research / Invited
• [Presentation] 多価不飽和脂肪酸の培地添加がラット培養心筋細胞の拍動収縮能に及ぼす効果 (2016)
  • Author(s): 小坂薫，井口 聖也，佐藤大介，馮 忠剛，楠正隆，中村孝夫
  • Organizer: SICE ライフエンジニアリング部門シンポジウム2016
  • Place of Presentation: 大阪国際交流センター（大阪）