| Project/Area Number |
16K12874
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Biomedical engineering/Biomaterial science and engineering
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
金子 新 京都大学, iPS細胞研究所, 准教授 (40361331)
三嶋 雄太 京都大学, iPS細胞研究所, 特定研究員 (80770263)
|
|---|
| Project Period (FY) |
2016-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | Organ-on-a-chip / マイクロ流体デバイス / iPS細胞 / 胸腺 / 血管 / T細胞 / 3次元培養 / organ-on-a-chip / マイクロデバイス / 生体模倣 / マイクロ・ナノデバイス / 再生医学 / システムオンチップ / 免疫学 / バイオテクノロジー |
|---|
| Outline of Final Research Achievements |
The goal of this study is to develop a culture device to mimic the function of the thymus which is necessary to form functional T cells. Using human induced pluripotent stem cell (hiPSC)-derived endothelial cells, we have successfully engineered a 3D vascular network in a hydrogel embedded within a microfluidic device. The engineered vascular network has open lumina connected to microchannels, which can deliver reagents and cells. Using this technique, we recapitulated a thymus-like microenvironment by culturing 3D cellular constructs containing thymus-like stromal cells with hiPSC-derived endothelial cells. This system enabled to perfuse blood cells through blood vessels connected to the tissue-like 3D cell constructs and to maintain blood cells inside the device. Thus, we have successful developed a microfluidic device to mimic the structure and microenvironment of the thymus.
|
