Induction of cardiomyocyte differenciation using a nano-particle based artificial transcription factor
Project/Area Number |
16K12896
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Biomedical engineering/Biomaterial science and engineering
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Research Institution | Kyoto University |
Principal Investigator |
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Project Period (FY) |
2016-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | ピロールイミダゾールポリアミド / 人工転写因子 / 金ナノ粒子 / 分化誘導 / 遺伝子・核酸工学材料 / 遺伝子制御分子 / 細胞リプログラミング / 心筋細胞 / ミトコンドリア / 人工ヒストンコード / ナノテクノロジー / リプログラミング |
Outline of Final Research Achievements |
Harnessing the chemical biology of nucleic acids, our aim was to successfully carry out bow-arrow reprogramming to achieve clinically useful cell types like cardiomyocytes (CMs) by switching ON and OFF the key transcription factors (TFs) using artificial TFs. We have successfully achieved the first-ever synthetic DNA-binding inhibitor of SOX2, which not only suppressed the pluripotency program in the hiPSCs but also activated the cardiac mesoderm program to generate functional spontaneously contracting CMs when supplemented with a Wnt-signalling inhibitor. Considering the presence of numerous mitochondria in CMs, we also created a ligand for mitochondrial gene modulation. Also, we programmed synthetic molecular codes capable of inducing targeted transcriptional activation of therapeutically important developmental genes in human somatic cells. Current studies with NANO-TFs, the nanoparticle version of synthetic-TFs and distinct bioactive peptides suggest enhanced bioefficacy.
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Report
(3 results)
Research Products
(30 results)
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[Presentation] Smart genetic switches2016
Author(s)
Ganesh N. Pandian
Organizer
Madurai Kamaraj University Visiting Program
Place of Presentation
Madurai Kamaraj University, Madurai, India
Year and Date
2016-10-30
Related Report
Invited
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[Presentation] Nature-inspired smart genetic switches2016
Author(s)
Ganesh N. Pandian
Organizer
7th Indian JSPS Alumni Association (IJAA) International Conference on Science and Technology: Future Challenges and Solutions (STFCS-2016)
Place of Presentation
University of Mysore, Mysore, India
Year and Date
2016-08-08
Related Report
Int'l Joint Research / Invited
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[Presentation] Epigenetics2016
Author(s)
Ganesh N. Pandian
Organizer
Department of Biotechnology Scientist of the Month Program
Place of Presentation
DG Vaishnav College, Chennai, India
Year and Date
2016-08-01
Related Report
Invited
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