Development of serum-free medium and Its application for basic and clinical research
| Project/Area Number |
16K12897
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Kagawa University |
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Principal Investigator |
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| Research Collaborator |
Yamashita Kayoko 香川大学, 医学研究科, 学生
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 血清 / 無血清 / FBS / 脂肪酸 / 増殖因子 / アルブミン / ピルビン酸 / インスリン / 脂質 / コレステロール / 培地 / リポタンパク質 / メタボローム |
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| Outline of Final Research Achievements |
In this research, we analyzed and profiled factors of the cell growth included in an animal serum, and I had development of the basic culture medium which can substitute for fetal calf serum for my object. Proliferation of T-cell lines, Jurkat, and Molt-4 was examined as model cells, and then, I found that insulin, transferrin, selenious acid (ITS) and lipoproteins are effective in a cell growth. I also found that existence of albumin which contains fatty acids is indispensable. A possibility of a novel growth factor that it may combine with the albumin & fetuin which exist much in the serum was found. The high growth-promoting effect was observed by the pyruvic acid, which is a well known effective substance in the organic acids, and oleic acid was effective in the fatty acids.
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Report
(3 results)
Research Products
(1 results)
