| Project/Area Number |
16K12904
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Kawasaki Institute of Industrial Promotion Innovation Center of NanoMedicine |
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Principal Investigator |
Quader Sabina 公益財団法人川崎市産業振興財団(ナノ医療イノベーションセンター), 川崎市産業振興財団, 主任研究員 (90749699)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | Neomycin / PolyIionComplex / siRNA / polymer / Aminoglycoside / Polyion Complex / cancer / Nucleic Acid / RNA-binding / aminoglycosides / PIC micelle / anticancer / combination-therapy |
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| Outline of Final Research Achievements |
The aim of this research is to construct PolyIonComplex (PIC), where, a widely used amimoglycoside antibiotic Neomycin, that has specific RNA-binding ability and also has anti-angiogenin property, will be used as polycationic segments to hold polyanionic siRNA or other nucleic acids for cancer-therapy. To achieve this purpose we designed a novel polymer conjugated with azide functionalized neomycin through click chemistry between the azide function of neomycin and alkyne function of polymer. Alkyne function was introduced in poly(ethylene glycol)-b-poly(β-benzyl-aspartamide) polymer via aminolysis reaction utilizing propargyl amine. We utilized dual armed PEG with each arm having molecular weight of around 40 KD and with only 6 repeating units of poly amino acid segments. This polymer was designed to conjugate calculated no of Neomycin units so to construct PolyIonComplex with minimum molar ratio of polymer and thus ultimately achieve very small size (around 25 nm) PIC.
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