Exploiting Neomycin-RNA complexation to create PIC micelle based efficient transportation for therapeutic siRNA
| Project/Area Number |
16K12904
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Kawasaki Institute of Industrial Promotion Innovation Center of NanoMedicine |
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Principal Investigator |
Quader Sabina 公益財団法人川崎市産業振興財団(ナノ医療イノベーションセンター), 川崎市産業振興財団, 主任研究員 (90749699)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | Neomycin / PolyIionComplex / siRNA / polymer / Aminoglycoside / Polyion Complex / cancer / Nucleic Acid / RNA-binding / aminoglycosides / PIC micelle / anticancer / combination-therapy |
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| Outline of Final Research Achievements |
The aim of this research is to construct PolyIonComplex (PIC), where, a widely used amimoglycoside antibiotic Neomycin, that has specific RNA-binding ability and also has anti-angiogenin property, will be used as polycationic segments to hold polyanionic siRNA or other nucleic acids for cancer-therapy. To achieve this purpose we designed a novel polymer conjugated with azide functionalized neomycin through click chemistry between the azide function of neomycin and alkyne function of polymer. Alkyne function was introduced in poly(ethylene glycol)-b-poly(β-benzyl-aspartamide) polymer via aminolysis reaction utilizing propargyl amine. We utilized dual armed PEG with each arm having molecular weight of around 40 KD and with only 6 repeating units of poly amino acid segments. This polymer was designed to conjugate calculated no of Neomycin units so to construct PolyIonComplex with minimum molar ratio of polymer and thus ultimately achieve very small size (around 25 nm) PIC.
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Report
(2 results)
Research Products
(8 results)
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[Journal Article] Proteasome inhibitor loaded micelles enhance antitumor activity through macrophage reprograming by NF-κB inhibition"2017
Author(s)
Hailiang Wu, Anqi Tao, John D. Martin, Sabina Quader, Xueying Liu, Kei Takahashi, Louise Hespel, Yutaka Miura, Yoshihiro Hayakawa, Tatsuro Irimura, Horacio Cabral, Kazunori Kataoka
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Journal Title
J. Pharm. Sci,
Volume: -
Issue: 9
Pages: 30217-30224
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Effective treatment of drug resistant recurrent breast tumors harboring cancer stem-like cells by staurosporine/epirubicin co-loaded polymeric micelles2017
Author(s)
J. Zhang, H. Kinoh, L. Hespel, X. Liu, S. Quader, J. Martin, T. Chida, H. Cabral, K. Kataoka,
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Journal Title
Journal of Controlled Release, "Vol. 264, pp. 127-1335, 2017
Volume: 264
Pages: 127-135
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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