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Re-endothelialization of decellularized coronary artery for cardiac regeneration

Research Project

Project/Area Number 16K12905
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biomedical engineering/Biomaterial science and engineering
Research InstitutionNational Cardiovascular Center Research Institute

Principal Investigator

YAMAOKA TETSUJI  国立研究開発法人国立循環器病研究センター, 研究所, 部長 (50243126)

Co-Investigator(Kenkyū-buntansha) 馬原 淳  国立研究開発法人国立循環器病研究センター, 研究所, 研究員 (80416221)
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords細胞・細胞工業材料 / 細胞・細胞工学材料
Outline of Final Research Achievements

The rat and minipig hearts were decellularized by SDS method or ultrahigh pressure (HP) method, and the decellularization efficiency was confirmed by HE staining and DNA quantification method. Since the SDS-based decellularized heart was much more transparent than that of HP method and then used for cell seeding experiments. The luminal surface of the coronary artery was modified with the endothelial cell-binding peptide, and suspension of Qdot-labeled HUVEC was perfused from the ascending aorta side to the apical side for a predetermined period. Three-dimensional observation by CLMS revealed that HUVECs were seeded not only into the main coronary arteries but also into more peripheral arteries by optimizing modification conditions and cell perfusion conditions.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (21 results)

All 2018 2017 2016

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (11 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Book (5 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 1 results)

  • [Journal Article] Design of in situ porcine closed-circuit system for assessing blood-contacting biomaterials2018

    • Author(s)
      M. C. Munisso, A. Mahara, T. Yamaoka
    • Journal Title

      Journal of Artificial Organs

      Volume: 印刷中 Issue: 3 Pages: 317-324

    • DOI

      10.1007/s10047-018-1042-5

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vivo guided vascular regeneration with a non-porous elastin-like polypeptide hydrogel tubular scaffold2017

    • Author(s)
      A. Mahara, K. L. Kiick, and T. Yamaoka
    • Journal Title

      Journal of Biomaterials Research: Part A

      Volume: 105 Issue: 6 Pages: 1746-1755

    • DOI

      10.1002/jbm.a.36018

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Novel peptides for small-caliber graft functionalization selected by a phage display of endothelial-positive/platelet-negative combined selection2017

    • Author(s)
      M. C. Munisso, T. Yamaoka
    • Journal Title

      Journal of Materials Chemistry B

      Volume: 5 Issue: 47 Pages: 9354-9364

    • DOI

      10.1039/c7tb02652h

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Presentation] 組織再生誘導型小口径脱細胞血管の長期移植と組織応答2018

    • Author(s)
      馬原淳
    • Organizer
      第17回日本再生医療学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 血管内皮細胞を選択的に誘導する脱細胞血管の新規修飾法2018

    • Author(s)
      古島健太郎
    • Organizer
      第17回日本再生医療学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 組織再生誘導型小口径脱細胞血管の開発1~ 内膜の再生誘導を狙ったリガンドペプチドの役割2017

    • Author(s)
      馬原 淳
    • Organizer
      第16回日本再生医療学会総会
    • Place of Presentation
      仙台国際センター(宮城県仙台市)
    • Year and Date
      2017-03-07
    • Related Report
      2016 Research-status Report
  • [Presentation] ブタ心臓の脱細胞化と冠動脈再内皮化2017

    • Author(s)
      森岡 聖文
    • Organizer
      第16回日本再生医療学会総会
    • Place of Presentation
      仙台国際センター(宮城県仙台市)
    • Year and Date
      2017-03-07
    • Related Report
      2016 Research-status Report
  • [Presentation] REDVペプチドを介した細胞ホーミングのリアルタイム観察2017

    • Author(s)
      北川和宜
    • Organizer
      日本バイオマテリアル学会関西ブロック 第12回関西若手研究発表会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 血中循環内皮前駆細胞によるペプチド修飾脱細胞血管の早期内膜形成2017

    • Author(s)
      馬原 淳
    • Organizer
      第55回日本人工臓器学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 最小口径の脱細胞化血管の要求特性解明と長期開存例2017

    • Author(s)
      山岡哲二
    • Organizer
      第66回高分子討論会
    • Related Report
      2017 Annual Research Report
  • [Presentation] REDV固定化表面上での血管内皮前駆細胞ホーミングの定量解析2017

    • Author(s)
      北川和宜
    • Organizer
      第39回日本バイオマテリアル学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] シランカップリング修飾剤を用いた組織再生型脱細胞血管への細胞親和性付与2017

    • Author(s)
      古島 健太郎
    • Organizer
      第39回日本バイオマテリアル学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Novel blood compatible surfaces for cardiovascular biomaterials2016

    • Author(s)
      山岡哲二
    • Organizer
      2nd Bone and Biomaterials workshop
    • Place of Presentation
      Inari, Finland
    • Year and Date
      2016-08-07
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] EPC capturing technology for improving patency of small-diameter(2mm ID) and long(30cm length) acellular blood vessels2016

    • Author(s)
      山岡哲二
    • Organizer
      3rd International Symposium on Advances in Sustainable Polymers
    • Place of Presentation
      京都工芸繊維大学(京都府京都市)
    • Year and Date
      2016-08-04
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Book] バイオマテリアル -生体材料― 36(2)2018

    • Author(s)
      山岡哲二
    • Total Pages
      8
    • Publisher
      田中昭文堂印刷
    • Related Report
      2017 Annual Research Report
  • [Book] 月刊「化学工業」 68(5)2017

    • Author(s)
      馬原 淳、山岡哲二
    • Total Pages
      4
    • Publisher
      化学工業社
    • Related Report
      2017 Annual Research Report
  • [Book] 医療・診断をささえるペプチド科学―再生医療・DDS・診断への応用―2017

    • Author(s)
      馬原 淳・山岡哲二
    • Total Pages
      8
    • Publisher
      CMC出版
    • ISBN
      9784781312675
    • Related Report
      2017 Annual Research Report
  • [Book] 日本経済新聞2017年2月7日2017

    • Author(s)
      山岡哲二
    • Total Pages
      1
    • Publisher
      日本経済新聞
    • Related Report
      2016 Research-status Report
  • [Book] 人工臓器45巻1号2016

    • Author(s)
      山岡哲二
    • Total Pages
      5
    • Publisher
      人工臓器
    • Related Report
      2016 Research-status Report
  • [Patent(Industrial Property Rights)] 血管内皮系細胞に特異的に結合するペプチドの使用、及びペプチド2018

    • Inventor(s)
      山岡哲二、マリアムニッソ、馬原淳、山本敬史
    • Industrial Property Rights Holder
      山岡哲二、マリアムニッソ、馬原淳、山本敬史
    • Industrial Property Rights Type
      特許
    • Filing Date
      2018
    • Related Report
      2017 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 血管内皮系細胞に特異的に結合するペプチドの使用、及びペプチド2017

    • Inventor(s)
      山岡哲二、マリアムニッソ、馬原淳、山本敬史
    • Industrial Property Rights Holder
      山岡哲二、マリアムニッソ、馬原淳、山本敬史
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2017-014800
    • Filing Date
      2017
    • Related Report
      2017 Annual Research Report

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Published: 2016-04-21   Modified: 2019-03-29  

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