• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Behavioral and electrophysiological analyses of new Alzheimer's disease model mouse that express amyloid beta oligomer intraneuronally

Research Project

Project/Area Number 16K12944
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Rehabilitation science/Welfare engineering
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

OCHIISHI TOMOYO  国立研究開発法人産業技術総合研究所, 生命工学領域, 主任研究員 (30356729)

Co-Investigator(Kenkyū-buntansha) 角 正美  植草学園大学, 保健医療学部, 講師 (30646261)
Research Collaborator Ebihara Tatsuhiko  
Kiyosue Kazuyuki  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsアルツハイマー病 / アミロイドβタンパク質 / シナプス / オリゴマー / トランスジェニックマウス / 運動 / GFP / 行動解析 / 習慣的運動 / 神経科学 / 脳神経疾患 / 老化
Outline of Final Research Achievements

Soluble amyloid β protein (Aβ) oligomers in neurons are neurotoxic and play crucial roles in synaptic transmission and cognitive function in Alzheimer’s disease (AD) patients. In this study, using GFP-fused human Aβ, which only forms low molecular weight oligomers, we developed a new mouse model that expresses Aβ oligomers throughout their life. They exhibit memory and long-term potentiation impairments already in young age. However, these mice showed improvement in cognitive function due to the spontaneous load of running wheel for about 2 months. Thus, the Aβ-GFP Tg mouse is a unique tool specialized to investigate the toxicity of Aβ oligomers inside neurons and the effect of exercise against the impairment of cognitive function induced by Aβ oligomer.

Academic Significance and Societal Importance of the Research Achievements

高齢化社会とあいまって、認知症の半数以上を占めるADの原因解明、治療法の確立は社会的要請となっている。MCIの患者における習慣的な運動の効果のメカニズムの解明は、認知症の予防や運動が効果を示す病理学的な時期の予想、また、非薬物的介入のみでなく運動による効果を高める物質等、薬物的治療法の開発など全く新しい治療法の開発に大きく貢献することが期待できる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (7 results)

All 2018 2017 2016 Other

All Presentation (5 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Book (1 results) Remarks (1 results)

  • [Presentation] Behavioral and electrophysiological analyses of new Alzheimer’s model mouse that express amyloid β oligomer intraneuronally.2018

    • Author(s)
      Tomoyo Ochiishi, Masami Kaku, Kazuyuki Kiyosue, Motomichi Doi, Tatsuhiko Ebihara
    • Organizer
      Neuroscience 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of the new animal model of Alzheimer’s disease that express the amyloid β oligomer.2017

    • Author(s)
      落石知世
    • Organizer
      2nd International conference on Nutraceuticals and Chronic Disease
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Behavioral and electrophysiological analysis of the Alzheimer’s model mouse the express amyloid β oligomer.2017

    • Author(s)
      落石知世、清末 和之、戸井 基道、海老原 達彦、角正美
    • Organizer
      生命科学系学会合同年次大会
    • Related Report
      2017 Research-status Report
  • [Presentation] アミロイドβオリゴマーを発現する新規アルツハイマーモデルマウスの解析2016

    • Author(s)
      落石知世、角正美、清末和之、志村秀樹、卜部貴夫、服部信孝、戸井基道、海老原達彦
    • Organizer
      第35回日本認知症学会学術集会
    • Place of Presentation
      東京国際フォーラム(東京都)
    • Year and Date
      2016-12-02
    • Related Report
      2016 Research-status Report
  • [Presentation] Behavioral and electrophysiological analysis of the Alzheimer’s disease model mouse that express amyloid β oligomer intraneuronally.2016

    • Author(s)
      Tomoyo Ochiishi, Kazuyuki Kiyosue, Masami Kaku, Motomichi Doi, Tatsuhiko Ebihara
    • Organizer
      第39回日本神経科学学会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2016-07-21
    • Related Report
      2016 Research-status Report
  • [Book] アルツハイマー病 発症メカニズムと新規診断法・創薬・治療開発2018

    • Author(s)
      落石知世、戸井基道
    • Total Pages
      460
    • Publisher
      株式会社 エヌ・ティー・エス
    • Related Report
      2018 Annual Research Report
  • [Remarks] 産総研公式HP(バイオメディカル研究部門)

    • URL

      https://unit.aist.go.jp/bmd/gr/index.html

    • Related Report
      2016 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi