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Elucidation of chromatin dynamics by an energy sensor histone demethylase

Research Project

Project/Area Number 16K13042
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Applied health science
Research InstitutionThe University of Tokyo

Principal Investigator

SAKAI Juro  東京大学, 先端科学技術研究センター, 教授 (80323020)

Co-Investigator(Renkei-kenkyūsha) KAWAMURA Takeshi  東京大学, アイソトープ総合センター, 准教授 (70306835)
KIMURA Hiroshi  東京工業大学, 生命理工学研究科, 教授 (30241392)
ABURATANI Hiroyuki  東京大学, 先端科学技術研究センター, 教授 (10202657)
INAGAKI Takeshi  群馬大学, 生体調節研究所, 教授 (10507825)
MATSUMURA Yoshihiro  東京大学, 先端科学技術研究センター, 准教授 (20375257)
ABE Yohei  東京大学, 先端科学技術研究センター, 学振特別研究員 (80771063)
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsヒストン脱メチル化酵素 / 栄養 / AMPキナーゼ / 飢餓時のエピゲノム変化 / 生活習慣病
Outline of Final Research Achievements

AMP Activated Protein Kinase (AMPK) is a serine / threonine phosphorylating enzyme and plays an extremely important role in metabolism, cell proliferation and reprogramming as an intracellular energy sensor. Since histone demethylase JMJD1A has multiple motifs of AMPK from “ScanSite”, we analyzed whether AMPK phosphorylated JMJD1A and was functioning. The JMJD1A recombinant protein was found to be phosphorylated AMP dependent in vitro by Phos-tag gel separation and immunoblotting (IB) with JMJD1A antibody and IB analysis using AMPK substrate antibody. Furthermore, phospho-proteomic analysis demonstrated that there are more than ten serine and/or threonine residues phosphorylated by AMPK. Identification of phosphorylated amino acid residues of endogenous JMJD1A by AMPK is still on the way.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (14 results)

All 2018 2017 2016 Other

All Journal Article (5 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 5 results,  Open Access: 1 results) Presentation (8 results) (of which Invited: 1 results) Remarks (1 results)

  • [Journal Article] Histone demethylase JMJD1A coordinates acute and chronic adaptation to cold stress via thermogenic phospho-switch.2018

    • Author(s)
      Yohei Abe, Yosuke Fujiwara, Hiroki Takahashi, Yoshihiro Matsumura, Tomonobu Sawada, Shuying Jiang, Ryo Nakaki, Aoi Uchida, Noriko Nagao, Makoto Naito, Shingo Kajimura, Hiroshi Kimura, Timothy F. Osborne, Hiroyuki Aburatani, Tatsuhiko Kodama, Takeshi Inagaki, Juro Sakai
    • Journal Title

      Nature Communications

      Volume: 9 Issue: 1 Pages: 1566-1566

    • DOI

      10.1038/s41467-018-03868-8

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] KDM3A coordinates actin dynamics with intraflagellar transport to regulate cilia stability.2017

    • Author(s)
      Yeyati PL, Schiller R, Mali G, Kasioulis I, Kawamura A, Adams IR, Playfoot C, Gilbert N, van Heyningen V, Wills J, von Kriegsheim A, Finch A, Sakai J, Schofield CJ, Jackson IJ, Mill P.
    • Journal Title

      J Cell Biol.

      Volume: 216 Issue: 4 Pages: 999-1013

    • DOI

      10.1083/jcb.201607032

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Degradation of human Lipin-1 by BTRC E3 ubiquitin ligase2017

    • Author(s)
      Ishimoto Kenji、Hayase Ayaka、Kumagai Fumiko、Kawai Megumi、Okuno Hiroko、Hino Nobumasa、Okada Yoshiaki、Kawamura Takeshi、Tanaka Toshiya、Hamakubo Takao、Sakai Juro、Kodama Tatsuhiko、Tachibana Keisuke、Doi Takefumi
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 488 Issue: 1 Pages: 159-164

    • DOI

      10.1016/j.bbrc.2017.04.159

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transcriptional and epigenetic control of brown and beige adipose cell fate and function.2017

    • Author(s)
      Inagaki T, Sakai J, Kajimura S.
    • Journal Title

      Nat Rev Mol Cell Biol.

      Volume: 18 Issue: 8 Pages: 527-527

    • DOI

      10.1038/nrm.2017.72

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Impact of histone demethylase KDM3A-dependent AP-1 transactivity on hepatotumorigenesis induced by PI3K activation2017

    • Author(s)
      Nakatsuka T, Tateishi K, Kudo Y, Yamamoto K, Nakagawa H, Fujiwara H, Takahashi R, Miyabayashi K, Asaoka Y, Tanaka Y, Ijichi H, Hirata Y, Otsuka M, Kato M, Sakai J, Tachibana M, Aburatani H, Shinkai Y, *Koike K.
    • Journal Title

      Oncogene

      Volume: 36 Issue: 45 Pages: 6262

    • DOI

      10.1038/onc.2017.222

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Presentation] ヒストン脱メチル化酵素による脂肪細胞の熱産生機構と糖代謝制御機構2018

    • Author(s)
      酒井寿郎
    • Organizer
      第52回糖尿病学の進歩
    • Related Report
      2017 Annual Research Report
  • [Presentation] 脂肪細胞における代謝産物とエピゲノムによるエネルギー代謝調節2017

    • Author(s)
      Eko F. Ariyanto, 松村欣宏, 曽我朋義, 稲垣毅, 酒井寿郎
    • Organizer
      日本がん分子標的治療学会 第12回トランスレーショナルリサーチワークショップ
    • Place of Presentation
      都市センターホテル(東京都・千代田区)
    • Year and Date
      2017-01-17
    • Related Report
      2016 Research-status Report
  • [Presentation] ヒストン脱メチル化酵素のリン酸化と脱メチル化による白色脂肪細胞の褐色化機構2017

    • Author(s)
      酒井寿郎
    • Organizer
      第11回エピジェネティクス研究会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] エピゲノムを介した白色脂肪細胞分化の制御機構2017

    • Author(s)
      松村欣宏, 仲木竜, 稲垣毅, 油谷浩幸, 酒井寿郎
    • Organizer
      第35回内分泌学代謝学サマーセミナー
    • Related Report
      2017 Annual Research Report
  • [Presentation] 脂肪細胞を特徴づける代謝によるエピゲノム制御2017

    • Author(s)
      松村欣宏, Eko Fuji Ariyanto, 曽我朋義, 酒井寿郎
    • Organizer
      2017年度生命科学系学会合同年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 寒冷刺激による翻訳後修飾とエピゲノム変化による脂肪細胞のベージュ化機構2017

    • Author(s)
      酒井寿郎, 阿部陽平, 藤原庸右, 高橋宙大, 児玉龍彦, 油谷浩幸, 松村欣宏, 稲垣毅
    • Organizer
      2017年度生命科学系学会合同年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ヒストンメチル化酵素SETDB1のユビキチン化は細胞内局在を制御する2016

    • Author(s)
      松村欣宏, 鹿野優佳, 吉田文乃, 川村猛, 稲垣毅, 酒井寿郎
    • Organizer
      第89回日本生化学会
    • Place of Presentation
      仙台国際センター(宮城県・仙台市)
    • Year and Date
      2016-09-26
    • Related Report
      2016 Research-status Report
  • [Presentation] 絶食でのケトン体産生のメカニズム2016

    • Author(s)
      酒井寿郎
    • Organizer
      第16回日本抗加齢医学会総会 シンポジウム
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2016-06-10
    • Related Report
      2016 Research-status Report
    • Invited
  • [Remarks] 東京大学先端科学技術研究センター酒井研究室/原著論文

    • URL

      http://www.mm.rcast.u-tokyo.ac.jp/publications/pubs.html

    • Related Report
      2017 Annual Research Report 2016 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2019-03-29  

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