Elucidation of chromatin dynamics by an energy sensor histone demethylase
| Project/Area Number |
16K13042
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied health science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SAKAI Juro 東京大学, 先端科学技術研究センター, 教授 (80323020)
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| Co-Investigator(Renkei-kenkyūsha) |
KAWAMURA Takeshi 東京大学, アイソトープ総合センター, 准教授 (70306835)
KIMURA Hiroshi 東京工業大学, 生命理工学研究科, 教授 (30241392)
ABURATANI Hiroyuki 東京大学, 先端科学技術研究センター, 教授 (10202657)
INAGAKI Takeshi 群馬大学, 生体調節研究所, 教授 (10507825)
MATSUMURA Yoshihiro 東京大学, 先端科学技術研究センター, 准教授 (20375257)
ABE Yohei 東京大学, 先端科学技術研究センター, 学振特別研究員 (80771063)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | ヒストン脱メチル化酵素 / 栄養 / AMPキナーゼ / 飢餓時のエピゲノム変化 / 生活習慣病 |
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| Outline of Final Research Achievements |
AMP Activated Protein Kinase (AMPK) is a serine / threonine phosphorylating enzyme and plays an extremely important role in metabolism, cell proliferation and reprogramming as an intracellular energy sensor. Since histone demethylase JMJD1A has multiple motifs of AMPK from “ScanSite”, we analyzed whether AMPK phosphorylated JMJD1A and was functioning. The JMJD1A recombinant protein was found to be phosphorylated AMP dependent in vitro by Phos-tag gel separation and immunoblotting (IB) with JMJD1A antibody and IB analysis using AMPK substrate antibody. Furthermore, phospho-proteomic analysis demonstrated that there are more than ten serine and/or threonine residues phosphorylated by AMPK. Identification of phosphorylated amino acid residues of endogenous JMJD1A by AMPK is still on the way.
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Report
(3 results)
Research Products
(14 results)
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[Journal Article] Histone demethylase JMJD1A coordinates acute and chronic adaptation to cold stress via thermogenic phospho-switch2018
Author(s)
Yohei Abe, Yosuke Fujiwara, Hiroki Takahashi, Yoshihiro Matsumura, Tomonobu Sawada, Shuying Jiang, Ryo Nakaki, Aoi Uchida, Noriko Nagao, Makoto Naito, Shingo Kajimura, Hiroshi Kimura, Timothy F. Osborne, Hiroyuki Aburatani, Tatsuhiko Kodama, Takeshi Inagaki, Juro Sakai
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Journal Title
Nature communications
Volume: 9
Pages: 1566-1566
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] KDM3A coordinates actin dynamics with intraflagellar transport to regulate cilia stability.2017
Author(s)
Yeyati PL, Schiller R, Mali G, Kasioulis I, Kawamura A, Adams IR, Playfoot C, Gilbert N, van Heyningen V, Wills J, von Kriegsheim A, Finch A, Sakai J, Schofield CJ, Jackson IJ, Mill P.
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Journal Title
J Cell Biol.
Volume: 216
Pages: 999-1013
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Impact of histone demethylase KDM3A-dependent AP-1 transactivity on hepatotumorigenesis induced by PI3K activation.2017
Author(s)
Nakatsuka T, Tateishi K, Kudo Y, Yamamoto K, Nakagawa H, Fujiwara H, Takahashi R, Miyabayashi K, Asaoka Y, Tanaka Y, Ijichi H, Hirata Y, Otsuka M, Kato M, Sakai J, Tachibana M, Aburatani H, Shinkai Y, Koike K.
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Journal Title
Oncogene
Volume: 36
Pages: 6262-6271
DOI
Related Report
Peer Reviewed
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