Chemical biology of multifunctional transporter GTR1

• Project/Area Number: 16K13083
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomolecular chemistry
• Research Institution: Tohoku University
• Principal Investigator: Ueda Minoru 東北大学, 理学研究科, 教授 (60265931)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 植物ホルモン輸送体 / GTR1 / NPR family / 輸送体 / Arabidopsis thaliana / ジャスモン酸イソロイシン / ジベレリン / グルコシノレート / 植物二次代謝産物

Outline of Final Research Achievements

Spatiotemporal distributions of plant hormones are regulated strictly through corresponding transporter proteins. GTR1 was found first found as a transporter of a jasmonoyl isoleucine (JA-Ile) and belongs to a NPF family to which 53 member proteins belong. Surprisingly, further studies strongly suggested that GTR1 is a unique `multi-functional` transporter which can transport three different kinds of plant metabolites, JA-Ile, gibberelin, and glucosinolate, across the membrane with structure recognition. We also found that the homo-dimerization of GTR1 is responsible for glucosinolate transport and heterodimerization with other NPF family protein is responsible for the transport of JA-Ile and GA. This is the first example of dimerization-mediated switching of substrate for transporter.

Research Products

• [Journal Article] Dimerization of GTR1 regulates their plasma membrane localization (2017)
  • Author(s): Ishimaru Yasuhiro、Washiyama Kento、Oikawa Takaya、Hamamoto Shin、Uozumi Nobuyuki、Ueda Minoru
  • Journal Title: Plant Signaling & Behavior Volume: 12 Issue: 6 Pages: 1334749-1334749
  • DOI: 10.1080/15592324.2017.1334749
  • Peer Reviewed / Open Access
• [Journal Article] GTR1 is a jasmonic acid and jasmonoyl-l-isoleucine transporter in Arabidopsis thaliana. (2017)
  • Author(s): Ishimaru, Y., Oikawa, T., Suzuki, T., Takeishi, S., Matsuura, H., Takahashi, K., Hamamoto, S., Uozumi, N., Shimizu, T., Seo, M., Ohta, H., and Ueda, M.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 81 Issue: 2 Pages: 249-255
  • DOI: 10.1080/09168451.2016.1246174
  • Peer Reviewed