| Project/Area Number |
16K13087
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomolecular chemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
AOYAMA Takashi 京都大学, 化学研究所, 教授 (80202498)
TOYOOKA Kiminori 理化学研究所, 顕微鏡解析ユニット, 上級研究員 (10360596)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 二次代謝 / 脂質分泌 / 植物細胞 / 二次代謝産物 |
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| Outline of Final Research Achievements |
A challenge was made to characterize the ‘bulk transport’ system involved in the secretion of lipophilic metabolites. Plants secrete many different lipophilic compounds, like wax, cutin and suberins, while secondary metabolic lipids of smaller molecular weight are also often secreted out of plant cells. However, the molecular basis of the secretion, how lipophilic metabolites go across the plasma membrane, is still largely unknown.
I this study, cultured cells and hairy roots of Lithospermum erythrorhizon have been used as a model of lipid secretion system, in which lipophilic naphthoquinone pigments, shikonin derivatives, are secreted out of the cells. It has been shown by inhibitor experiments that the secretion process of shikonin, actin filaments and ARF/GEF are involved in a similar way as membrane trafficking of sec vesicles. Secreted lipid molecules out of shikonin derivatives have been also analyzed by LC-MS and GC-MS.
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