| Project/Area Number |
16K13088
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomolecular chemistry
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| Research Institution | Osaka University |
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Principal Investigator |
Hori Yuichiro 大阪大学, 工学研究科, 准教授 (00444563)
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| Research Collaborator |
NISHIURA Miyako
HIRAYAMA Shinya
OTOMURA Norimichi
NISHIDA Ayuko
MORI Kazuma
YAMASAKI Kohei
UMENO Maho
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | OFF-ON-OFFプローブ / PYPタグ / 蛋白質分解 / 分子力学計算 / タグ / ラベル化 / OFF-ON-OFF蛍光標識プローブ / OFF-ON-OFF蛍光スイッチ |
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| Outline of Final Research Achievements |
In this research, we developed protein labeling probes for imaging protein lifetime using our original PYP-tag labeling method and a new chemical principle based on OFF-ON-OFF fluorescence switching. The regulation of intramolecular association made the probes nonfluorescent in a free state, fluorescent in a PYP-tag-bound form and nonfluorescent after protein degradation. By using one of the probes, we imaged protein expression and degradation in living cells.
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