Fluorescence screening and imaging by chemical probes for detecting histone deacetylases/demethylases activity
Project/Area Number |
16K13099
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Chemical biology
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Research Institution | Osaka University |
Principal Investigator |
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Project Period (FY) |
2016-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
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Keywords | 蛍光プローブ / ヒストン脱メチル化酵素 / エピジェネティクス / ヒストン脱アセチル化酵素 / リジン脱メチル化酵素 / エピゲノム |
Outline of Final Research Achievements |
Fluorescent probes were developed for detection of lysine demethylase (KDM) activity. This fluorescent probe produced a fluorescent coumarin upon lysine demethylation by utilizing the intramolecular transesterification mechanism. As results, KDM activity was successfully detected with a single-step manipulation for the first time. The probe will be useful for understanding the role of KDM and contribute to development of drugs targeting KDM activity.
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Report
(2 results)
Research Products
(8 results)
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[Journal Article] Fabrication of ‘Clickable’ Polyfluorene Nanowires with High Aspect Ratio as Biological Sensing Platforms2016
Author(s)
T. Wasin, K. Enomoto, T. Sakurai, V. Padalkar, H. Cheng, M. Tang, A. Horio, D., Sakamaki, M. Omichi, A. Saeki, K. Kikuchi, Y. Hori, A. Chiba, Y. Saito, T. Kamiya, M. Sugimoto, S. Seki
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Journal Title
ACS Sensors
Volume: 6
Issue: 6
Pages: 766-774
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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