Research Project
Grant-in-Aid for Challenging Exploratory Research
Four kinds of deuterium-substituted fatty acids were chemically synthesized to prepare 2H-labeled sphingomyelin (SM) as a raft lipid and phosphatidylcholine (PC) as a control lipid. The results of 2H NMR and IR spectra of the labeled SM bilayer indicated that the melting temperature (mp) is depth-dependent with a lower mp for the terminal part of acyl chains while the melting of PC is less depth-dependent. Also, a clear melting point disappeared by an addition of cholesterol for both SM and PC membranes while the conformational change of SM terminal chains observed in IR spectra may account for the difference in the depth dependent melting of SM as compared with PC.
All 2018 2017 2016 Other
All Int'l Joint Research (1 results) Journal Article (2 results) (of which Int'l Joint Research: 1 results, Peer Reviewed: 2 results) Presentation (6 results) (of which Int'l Joint Research: 1 results, Invited: 3 results) Book (1 results) Remarks (2 results)
Biophysical Journal
Volume: 112 Issue: 5 Pages: 976-983
10.1016/j.bpj.2017.01.016
Biophys J.
Volume: 111 Issue: 3 Pages: 546-556
10.1016/j.bpj.2016.06.036
http://www.chem.sci.osaka-u.ac.jp/lab/murata/research.html
http://www.chem.sci.osaka-u.ac.jp/lab/murata/