Depth-dependent melting of acyl chains and phase-transition of lipid bilayers

• Project/Area Number: 16K13100
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Chemical biology
• Research Institution: Osaka University
• Principal Investigator: Murata Michio 大阪大学, 理学研究科, 教授 (40183652)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 脂質二重膜 / 相転移温度 / スフィンゴミエリン / フォスファチジルコリン / 重水素NMR / 赤外分光スペクトル / 重水素ＮＭＲ / リン脂質 / 化学プローブ

Outline of Final Research Achievements

Four kinds of deuterium-substituted fatty acids were chemically synthesized to prepare 2H-labeled sphingomyelin (SM) as a raft lipid and phosphatidylcholine (PC) as a control lipid. The results of 2H NMR and IR spectra of the labeled SM bilayer indicated that the melting temperature (mp) is depth-dependent with a lower mp for the terminal part of acyl chains while the melting of PC is less depth-dependent. Also, a clear melting point disappeared by an addition of cholesterol for both SM and PC membranes while the conformational change of SM terminal chains observed in IR spectra may account for the difference in the depth dependent melting of SM as compared with PC.

Research Products

• [Int'l Joint Research] Abo Akademi University(フィンランド)
• [Journal Article] The Long-Chain Sphingoid Base of Ceramides Determines Their Propensity for Lateral Segregation (2017)
  • Author(s): Al Sazzad Md. Abdullah、Yasuda Tomokazu、Murata Michio、Slotte J. Peter
  • Journal Title: Biophysical Journal Volume: 112 Issue: 5 Pages: 976-983
  • DOI: 10.1016/j.bpj.2017.01.016
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The Affinity of Cholesterol for Different Phospholipids Affects Lateral Segregation in Bilayers (2016)
  • Author(s): Oskar Engberg, Victor Hautala, Tomokazu Yasuda, Henrike Dehio, Michio Murata, J. Peter Slotte, Thomas K. M. Nyholm
  • Journal Title: Biophys J. Volume: 111 Issue: 3 Pages: 546-556
  • DOI: 10.1016/j.bpj.2016.06.036
  • Peer Reviewed
• [Presentation] 重水素化スフィンゴミエリンのアシル鎖の深度依存的な配座の赤 外分光解析とコレステロールの添加効果 (2018)
  • Author(s): 佐伯 直香・土川 博史・門司 真美・梅川 雄一・村田 道雄・J. Peter Slotte
  • Organizer: 日本化学会第９８春季年会
• [Presentation] リン脂質二重膜の動的構造 ー脂質ラフトとマイクロドメインー (2018)
  • Author(s): 村田道雄
  • Organizer: 大阪大学 蛋白研セミナー
  • Invited
• [Presentation] 細胞膜の情報伝達プラットフォーム・脂質ラフトを化学する (2017)
  • Author(s): 村田道雄
  • Organizer: 神奈川大学 有機化学の最前線
  • Invited
• [Presentation] Atomistic picture of lipid rafts in model membrane (2017)
  • Author(s): Michio Murata
  • Organizer: 6th Gratama Workshop at Groningen
  • Int'l Joint Research / Invited
• [Presentation] 脂質モデル膜における極長鎖スフィンゴミエリンの運動性と膜物性解析 (2017)
  • Author(s): 森内 舞・土川 博史・安田 智一・村田 道雄・Slotte J. Peter
  • Organizer: 日本化学会第97春季年会
  • Place of Presentation: 神奈川
• [Presentation] 固体重水素ＮＭＲ測定のための化学シフト温度計の開発と水和脂質二重膜への適用 (2017)
  • Author(s): 下西剛史, 梅川雄一, 門司真美, 花島慎弥, 土川博史, 村田 道雄
  • Organizer: 日本化学会第97春季年会
  • Place of Presentation: 神奈川
• [Book] Annual Reports on NMR Spectroscopy, Volume 94 (2018)
  • Author(s): Yuichi Umegawa, Nobuaki Matsumori and Michio Murata
  • Total Pages: 260
  • Publisher: Elsevier
  • ISBN: 9780128161166
• [Remarks] 研究紹介
  • URL: http://www.chem.sci.osaka-u.ac.jp/lab/murata/research.html
• [Remarks] 村田研究室
  • URL: http://www.chem.sci.osaka-u.ac.jp/lab/murata/