Development of Photostable Supramolecular Fluorophores and Application to Bioimaging
| Project/Area Number |
16K13102
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemical biology
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| Research Institution | Tohoku University |
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Principal Investigator |
MIZUKAMI Shin 東北大学, 多元物質科学研究所, 教授 (30420433)
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| Co-Investigator(Renkei-kenkyūsha) |
KIKUCHI KAZUYA 大阪大学, 大学院工学研究科, 教授 (70292951)
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| Research Collaborator |
IMOTO TAKUMA 大阪大学, 大学院工学研究科, 大学院生
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 超分子化学 / 蛍光色素 / 光退色 / 希土類イオン / 光安定性 / 超分子 / 生細胞イメージング |
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| Outline of Final Research Achievements |
Recently, microscopic imaging techniques with high intensity lasers such as single molecule imaging and super resolution imaging have attracted attention. However, one of the unsolved problems is photobleaching of fluorophores under the microscopic measurement. In this project, we developed new technologies to suppress photobleaching of fluorescent dyes.
In the first year, we investigated whether photobleaching can be suppressed by incorporating a fluorescent dye in a host molecule. The photobleaching was dramatically inhibited by an appropriate combination of a certain type of dye and host. In the second year, we discovered the anti-fading effect of some lanthanide ions. We also considered the anti-fading mechanism.
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Report
(3 results)
Research Products
(20 results)
