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Development of Photostable Supramolecular Fluorophores and Application to Bioimaging

Research Project

Project/Area Number 16K13102
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Chemical biology
Research InstitutionTohoku University

Principal Investigator

MIZUKAMI Shin  東北大学, 多元物質科学研究所, 教授 (30420433)

Co-Investigator(Renkei-kenkyūsha) KIKUCHI KAZUYA  大阪大学, 大学院工学研究科, 教授 (70292951)
Research Collaborator IMOTO TAKUMA  大阪大学, 大学院工学研究科, 大学院生
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords超分子化学 / 蛍光色素 / 光退色 / 希土類イオン / 光安定性 / 超分子 / 生細胞イメージング
Outline of Final Research Achievements

Recently, microscopic imaging techniques with high intensity lasers such as single molecule imaging and super resolution imaging have attracted attention. However, one of the unsolved problems is photobleaching of fluorophores under the microscopic measurement. In this project, we developed new technologies to suppress photobleaching of fluorescent dyes.
In the first year, we investigated whether photobleaching can be suppressed by incorporating a fluorescent dye in a host molecule. The photobleaching was dramatically inhibited by an appropriate combination of a certain type of dye and host. In the second year, we discovered the anti-fading effect of some lanthanide ions. We also considered the anti-fading mechanism.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (20 results)

All 2018 2017 2016

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (13 results) (of which Int'l Joint Research: 7 results,  Invited: 11 results)

  • [Journal Article] Ratiometric Imaging of Intracellular Mg2+ Dynamics Using a Red Fluorescent Turn-off Probe and a Green Fluorescent Turn-on Probe2018

    • Author(s)
      Matsui Yusuke、Mizukami Shin、Kikuchi Kazuya
    • Journal Title

      Chemistry Letters

      Volume: 47 Issue: 1 Pages: 23-26

    • DOI

      10.1246/cl.170918

    • NAID

      130006302062

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Visualization of long-term Mg2+ dynamics in apoptotic cells using a novel targetable fluorescent probe2017

    • Author(s)
      Matsui Y, Funato Y, Imamura H, Miki H, Mizukami S, Kikuchi K
    • Journal Title

      Chem Sci.

      Volume: 8 Issue: 12 Pages: 8255-8264

    • DOI

      10.1039/c7sc03954a

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Highly selective tridentate fluorescent probes for visualizing intracellular Mg2+ dynamics without interference from Ca2+ fluctuation2017

    • Author(s)
      Matsui Yusuke、Sadhu Kalyan K.、Mizukami Shin、Kikuchi Kazuya
    • Journal Title

      Chemical Communications

      Volume: 53 Issue: 77 Pages: 10644-10647

    • DOI

      10.1039/c7cc06141b

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Intracellular Protein-Labeling Probes for Multicolor Single-Molecule Imaging of Immune Receptor?Adaptor Molecular Dynamics2017

    • Author(s)
      Sato Ryota、Kozuka Jun、Ueda Masahiro、Mishima Reiko、Kumagai Yutaro、Yoshimura Akimasa、Minoshima Masafumi、Mizukami Shin、Kikuchi Kazuya
    • Journal Title

      Journal of the American Chemical Society

      Volume: 139 Issue: 48 Pages: 17397-17404

    • DOI

      10.1021/jacs.7b08262

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enzyme-Triggered Compound Release using Functionalized Antimicrobial Peptide Derivatives2017

    • Author(s)
      Shin Mizukami, Masayoshi Kashibe, Kengo Matsumoto, Yuichiro Hori, Kazuya Kikuchi
    • Journal Title

      Chem. Sci.

      Volume: 8 Issue: 4 Pages: 3047-3053

    • DOI

      10.1039/c6sc04435b

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Targetable Fluorescent Sensors for Advanced Cell Function Analysis2017

    • Author(s)
      Shin Mizukami
    • Journal Title

      J. Photochem. Photobiol. C

      Volume: 30 Pages: 24-35

    • DOI

      10.1016/j.jphotochemrev.2017.01.003

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Real-Time Intravital Imaging of pH Variation Associated with Osteoclast Activity2016

    • Author(s)
      Hiroki Maeda, Toshiyuki Kowada, Junichi Kikuta, Masayuki Furuya, Mai Shirazaki, Shin Mizukami, Masaru Ishii, Kazuya Kikuchi
    • Journal Title

      Nat. Chem. Biol.

      Volume: 12 Issue: 8 Pages: 579-585

    • DOI

      10.1038/nchembio.2096

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] 機能性分子局在化技術のバイオイメージングへの展開2018

    • Author(s)
      水上進
    • Organizer
      日本化学会第98春季年会特別企画
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 分子のレジデンス制御を設計指針とした生体解析プローブの開発2018

    • Author(s)
      水上進
    • Organizer
      日本化学会第98春季年会特別企画
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Development of Bioanalytical Technologies Based on Rational Design of Organic-Nano Hybrid Materials2017

    • Author(s)
      水上 進
    • Organizer
      第94回日本生理学会
    • Place of Presentation
      アクトシティ浜松(浜松)
    • Year and Date
      2017-03-28
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 有機蛍光色素の光安定性に及ぼす希土類イオン添加の効果2017

    • Author(s)
      井元琢真, 水上進, 菊地和也
    • Organizer
      日本化学会第96春季年会
    • Place of Presentation
      慶應大学(横浜)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] 機能性プローブ設計に基づく生体機能解析技術の開発2017

    • Author(s)
      水上 進
    • Organizer
      愛媛大学大学院医学研究科・分子病態医学セミナー
    • Place of Presentation
      愛媛大学(東温)
    • Year and Date
      2017-03-07
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 分子・材料の機能設計に基づく生体解析技術の開発2017

    • Author(s)
      水上進
    • Organizer
      第34回無機・分析化学コロキウム
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 低分子~ナノ粒子プローブによる生体内現象の可視化2017

    • Author(s)
      水上進
    • Organizer
      2017年光化学討論会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 機能性分子設計に基づく生体イメージング技術の開発2017

    • Author(s)
      水上進
    • Organizer
      ニコンイメージングセンター学術講演会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Strategy to improve photostability of fluorescent dyes by using lanthanide ion2016

    • Author(s)
      Takuma Imoto, Shin Mizukami, Kazuya Kikuchi
    • Organizer
      Labeling & Nanoscopy 2016
    • Place of Presentation
      ハイデルベルク(ドイツ)
    • Year and Date
      2016-10-30
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Development of bioanalytical tools based on organic-inorganic hybrid nanomaterials2016

    • Author(s)
      Shin Mizukami
    • Organizer
      HOKUDAI-NCTU International Joint Symposium on Nano, Opto and Bio Scinences
    • Place of Presentation
      北海道大学(札幌)
    • Year and Date
      2016-10-04
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Bioimaging and bioanalytical applications with middle molecules on nanaomaterials2016

    • Author(s)
      Shin Mizukami
    • Organizer
      Satellite International Mini Symposium on Middle Molecular Strategy in Sendai
    • Place of Presentation
      東北大学(仙台)
    • Year and Date
      2016-09-20
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Development of molecular imaging probes constructed of small molecules, proteins, and nanomaterials2016

    • Author(s)
      水上 進
    • Organizer
      化学系学協会東北大会
    • Place of Presentation
      いわき明星大学(いわき)
    • Year and Date
      2016-09-10
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Fluorescent probes with molecular targeting property2016

    • Author(s)
      Shin Mizukami
    • Organizer
      CWRU-Tohoku 3rd Joint workshop
    • Place of Presentation
      東北大学(仙台)
    • Year and Date
      2016-08-10
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited

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Published: 2016-04-21   Modified: 2019-03-29  

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