| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
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| Outline of Final Research Achievements |
We previously developed a genetically encoded calcium indicator RCaMP2, which surpasses performance obtained with classical genetically encoded or chemical dye-based calcium indicators. Transformative insights for rational design of R-CaMP2 were gained from previous biochemical research on calcium-calmodulin-binding proteins pioneered by the investigator group. In this study, we expanded this strategy, and rationally pursued protein engineering-based iterative mutation processes to realize multiple color calcium recording that goes beyond the classical two colors, green and red. Multicolor cellular activiy measurements represent an urgent technological problem to solve. Our approach opens a hitherto unexplored, brand-new, avenue for a novel fast multiplex calcium imaging platform through an original GECI design concept.
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