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Elucidation of antimicrobial mechanism by silver nanoparticles

Research Project

Project/Area Number 16K13628
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Nanomaterials chemistry
Research InstitutionOsaka University

Principal Investigator

Seino Satoshi  大阪大学, 工学研究科, 准教授 (90432517)

Research Collaborator IMOTO Yasuo  
NISHIDA Tomoki  
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords銀ナノ粒子 / 抗微生物 / ナノ材料 / ナノバイオ
Outline of Final Research Achievements

Antimicrobial mechanism of silver nanoparticles were discussed by using material analysis methods. The XAFS analysis technique was used to investigate the change in the chemical state of silver nanoparticles under antimicrobial test environments. Sulfur-containing substances such as cysteine, methionine and glutathione was used as model compounds. It was revealed that chemical state of the silver nanoparticles changed from the metal to form sulfur bonded state under high concentration of sulfur-containing substances. It was strongly suggested that this change in the chemical state is a factor to inactivate antimicrobial properties.
SEM observation of antibacterial treated bacteria was carried out using E-coli as the model bacterial species. It was revealed that the action mechanism of silver nanoparticles was not membrane destruction.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (15 results)

All 2018 2017 2016

All Journal Article (7 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Acknowledgement Compliant: 2 results) Presentation (8 results) (of which Invited: 1 results)

  • [Journal Article] 抗菌試験環境における銀ナノ粒子の化学状態と抗菌性能との関係2018

    • Author(s)
      豊田桃子, 清野智史, 射本康夫, 中嶋絵里, 中川貴, 山本孝夫
    • Journal Title

      日本防菌防黴学会誌

      Volume: in press

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 金属銀ナノ粒子の抗菌作用機構2018

    • Author(s)
      清野智史
    • Journal Title

      ケミカルエンジニヤリング

      Volume: 63 Pages: 293-298

    • Related Report
      2017 Annual Research Report
  • [Journal Article] 抗微生物機能付与を目的とした繊維への銀ナノ粒子固定化法の開発2018

    • Author(s)
      清野智史
    • Journal Title

      化学工学

      Volume: 82 Pages: 143-145

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Quantitative Methods for Testing Antiviral Activities of Textile Fabrics2017

    • Author(s)
      Y. Imoto, S. Seino, T. Nakagawa and T. A. Yamamoto
    • Journal Title

      Journal of Antimicrobial Agents

      Volume: 3 Issue: 03 Pages: 1000146-1000146

    • DOI

      10.4172/2472-1212.1000146

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 放射線を利用した独自ナノ粒子合成技術と大学発ベンチャーでの実用化2017

    • Author(s)
      清野智史
    • Journal Title

      大阪大学工業会誌 テクノネット

      Volume: 4 Pages: 10-15

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Comparison of Quantitative Antifungal Testing Methods for Textile Fabrics2017

    • Author(s)
      Yasuo Imoto, Satoshi Seino, Takashi Nakagawa, Takao A. Yamamoto
    • Journal Title

      Biocontrol Science

      Volume: 22 Pages: 47-53

    • NAID

      130005493105

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Antiviral Activity of Silver Nanoparticles Immobilized onto Textile Fabrics Synthesized by Radiochemical Process2016

    • Author(s)
      Satoshi Seino, Yasuo Imoto, Tomoya Kosaka, Tomoki Nishida, Takashi Nakagawa, Takao A. Yamamoto
    • Journal Title

      Materials Research Society Symposium Proceedings

      Volume: 印刷中 Issue: 11 Pages: 705-710

    • DOI

      10.1557/adv.2016.43

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] 抗菌試験環境における銀ナノ粒子の化学状態2018

    • Author(s)
      清野智史 豊田桃子 射本康夫 中川 貴  山本孝夫
    • Organizer
      第7回名古屋大学 シンクロトロン光研究センターシンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] 滅菌、消毒処理による細菌の形態変化観察2017

    • Author(s)
      射本康夫、西田倫希、清野智史、中川貴、山本孝夫
    • Organizer
      日本防菌防黴学会 第44回年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 放射線照射還元法による貴金属ナノ粒子担持材料の合成2017

    • Author(s)
      清野 智史、大久保 雄司、久貝 潤一郎、中川 貴、山本 孝夫
    • Organizer
      日本金属学会2017年秋期(第161回)講演大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 抗微生物試験環境における銀ナノ粒子の化学状態2017

    • Author(s)
      清野智史、豊田桃子、射本康夫、中川貴、山本孝夫
    • Organizer
      第20回XAFS討論会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 銀ナノ粒子の抗菌作用機構の検討2017

    • Author(s)
      豊田桃子、清野智史、射本康夫、西田倫希、中嶋絵里、中川貴、山本孝夫
    • Organizer
      ナノ学会第15回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 金属銀ナノ粒子の 抗菌性能と抗ウイルス性能2017

    • Author(s)
      清野智史
    • Organizer
      技術情報協会セミナー 抗菌・抗ウイルス性の付与技術と性能評価,開発事例
    • Related Report
      2017 Annual Research Report
  • [Presentation] 繊維製品の抗かび性定量試験方法の比較2016

    • Author(s)
      射本康夫、清野智史、中川貴、山本孝夫
    • Organizer
      日本防菌防黴学会 第43回年次大会
    • Place of Presentation
      東京都品川区・きゅりあん
    • Year and Date
      2016-09-26
    • Related Report
      2016 Research-status Report
  • [Presentation] 放射線還元法による繊維への金属銀ナノ粒子の固定化2016

    • Author(s)
      清野智史、射本康夫、中川 貴、山本孝夫
    • Organizer
      46.日本金属学会 2016年秋季(第159回)講演大会
    • Place of Presentation
      大阪大学
    • Year and Date
      2016-09-21
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2019-03-29  

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