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Measurement of enzymatic reaction using graphene and its application

Research Project

Project/Area Number 16K13638
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Nanomaterials engineering
Research InstitutionOsaka University

Principal Investigator

Ono Takao  大阪大学, 産業科学研究所, 助教 (00752875)

Project Period (FY) 2016-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Keywordsグラフェン / 酵素反応 / 電界効果トランジスタ / マイクロウェル / デバイ長 / ヘリコバクター・ピロリ / マイクロ・ナノデバイス / バイオ分析 / 酵素反応計測
Outline of Final Research Achievements

In this project, I developed a biosensing technology using "electrical detection of enzyme product by graphene transistor" and "encapsulation of reaction system in microdroplet". It is a first step of "Lab on a graphene", a novel platform for biosensing using graphene and resolves a problem derived from Debye screening. Enzyme urease was captured on graphene and its reaction product was successfully detected by graphene transistor. Also, pathogen was captured on graphene and detected by its reaction product. These results show my novel method can overcome the limitation of Debye screening and be applied to various kind of biosensing.

Report

(2 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • Research Products

    (10 results)

All 2017 2016 Other

All Journal Article (3 results) (of which Peer Reviewed: 2 results,  Acknowledgement Compliant: 2 results) Presentation (6 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Remarks (1 results)

  • [Journal Article] Glycan-functionalized graphene-FETs toward selective detection of human-infectious avian influenza virus2017

    • Author(s)
      Takao Ono
    • Journal Title

      Japanese Journal of Applied Physics

      Volume: 56 Issue: 3 Pages: 030302-030302

    • DOI

      10.7567/jjap.56.030302

    • NAID

      210000147449

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] グラフェントランジスタのバイオセンシング応用 -Lab on a graphene-2017

    • Author(s)
      小野尭生、金井康、奥田聡志、大野恭秀、前橋兼三、井上恒一、松本和彦
    • Journal Title

      Journal of The Surface Science Society of Japan

      Volume: 印刷中

    • NAID

      130005175850

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] グラフェンを用いたバイオセンシング―DNA検出からインフルエンザ診断まで―2016

    • Author(s)
      小野尭生、金井康、大野恭秀、前橋兼三、井上恒一、松本和彦
    • Journal Title

      MATERIAL STAGE

      Volume: 16 Pages: 65-70

    • NAID

      40021003549

    • Related Report
      2016 Annual Research Report
  • [Presentation] Measurement of Enzymatic Reaction using Graphene Field-Effect Transistor and Microwell for Detection of Helicobacter pylori2017

    • Author(s)
      Takao Ono, Yasushi Kanai, Yasuhide Ohno, Kenzo Maehashi, Koichi Inoue, and Kazuhiko Matsumoto
    • Organizer
      59th Electronic Materials Conference
    • Place of Presentation
      South Bend, Indiana (USA)
    • Year and Date
      2017-06-28
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マイクロウェルと複合化したグラフェントランジスタを用いたHelicobacter pyloriの検出2017

    • Author(s)
      小野尭生、金井康、大野恭秀、前橋兼三、井上恒一、松本和彦
    • Organizer
      第64回 応用物理学会 春季学術講演会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2017-03-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] グラフェントランジスタのバイオセンシング応用 -Lab on a graphene-2016

    • Author(s)
      小野尭生
    • Organizer
      第36回表面科学学術講演会・第57回真空に関する連合講演会
    • Place of Presentation
      名古屋国際会議場(愛知県名古屋市)
    • Year and Date
      2016-11-29
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Measurement of Enzyme Molecules and Their Reactions Using Graphene-FET Equipped with Microwells2016

    • Author(s)
      Takao Ono, Yasushi Kanai, Yasuhide Ohno, Kenzo Maehashi, Koichi Inoue, and Kazuhiko Matsumoto
    • Organizer
      2016 International Conference on Solid State Devices and Materials
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Year and Date
      2016-09-28
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] グラフェン電界効果トランジスタ上に捕捉した酵素分子の高感度検出2016

    • Author(s)
      小野尭生、金井康、大野恭秀、前橋兼三、井上恒一、松本和彦
    • Organizer
      第77回応用物理学会秋期学術講演会
    • Place of Presentation
      朱鷺メッセ(新潟県新潟市)
    • Year and Date
      2016-09-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] An Application of Graphene Field Effect Transistor to Enzymatic Assay2016

    • Author(s)
      Takao Ono, Yasushi Kanai, Yasuhide Ohno, Kenzo Maehashi, Koichi Inoue, and Kazuhiko Matsumoto
    • Organizer
      The 2016 Compound Semiconductor Week
    • Place of Presentation
      富山国際会議場(富山県富山市)
    • Year and Date
      2016-06-30
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Remarks] 産業科学研究所 松本研究室

    • URL

      http://www.sanken.osaka-u.ac.jp/labs/se/

    • Related Report
      2016 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2021-01-27  

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