Measurement of enzymatic reaction using graphene and its application
| Project/Area Number |
16K13638
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nanomaterials engineering
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| Research Institution | Osaka University |
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Principal Investigator |
Ono Takao 大阪大学, 産業科学研究所, 助教 (00752875)
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| Project Period (FY) |
2016-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | グラフェン / 酵素反応 / 電界効果トランジスタ / マイクロウェル / デバイ長 / ヘリコバクター・ピロリ / マイクロ・ナノデバイス / バイオ分析 / 酵素反応計測 |
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| Outline of Final Research Achievements |
In this project, I developed a biosensing technology using "electrical detection of enzyme product by graphene transistor" and "encapsulation of reaction system in microdroplet". It is a first step of "Lab on a graphene", a novel platform for biosensing using graphene and resolves a problem derived from Debye screening. Enzyme urease was captured on graphene and its reaction product was successfully detected by graphene transistor. Also, pathogen was captured on graphene and detected by its reaction product. These results show my novel method can overcome the limitation of Debye screening and be applied to various kind of biosensing.
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Report
(2 results)
Research Products
(10 results)
