Creation of non-spherical liposomes using a microdevice
| Project/Area Number |
16K13656
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Nano/Microsystems
|
|---|
| Research Institution | Kanagawa Institute of Industrial Sclence and Technology (2017)
Kanagawa Academy of Science and Technology (2016) |
|---|
Principal Investigator |
Kamiya Koki 地方独立行政法人神奈川県立産業技術総合研究所, 人工細胞膜システムグループ, 研究員(任期有) (70612315)
|
|---|
| Project Period (FY) |
2016-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | リポソーム / 細胞模倣膜 / 人工細胞モデル / マイクロデバイス / 非球体リポソーム / ベシクル / 人工細胞 / 非球面リポソーム / マイクロ・ナノデバイス / マイクロマシン / 脂質二分子膜 |
|---|
| Outline of Final Research Achievements |
I developed a cell-sized non-spherical liposome formation using three-dimensional micro structures to emulate a surface structure of cultured cells. I fabricated a micro-cubic frame based on a stackable photoresist processed using two photon laser writing. Next, I formed lipid membranes on the faces of the micro-cubic frame structures into microfluidic devices. Moreover, to confirm the formation of the lipid bilayer membranes on the faces of the micro-cubic frame structures, nanopore-forming membrane proteins were reconstituted into the non-spherical liposomes. I observed the diffusion of fluorescent molecules through the nanopore-forming membrane proteins.
|
Report
(3 results)
Research Products
(7 results)
