Driving myosin by polymorphism and hydration-state control of actin filaments
| Project/Area Number |
16K13860
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biological physics/Chemical physics/Soft matter physics
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| Research Institution | Tohoku University |
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Principal Investigator |
Suzuki Makoto 東北大学, 工学研究科, 教授 (60282109)
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| Co-Investigator(Kenkyū-buntansha) |
最上 譲二 東北大学, 工学研究科, 助教 (70713022)
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| Co-Investigator(Renkei-kenkyūsha) |
MATUBAYASI Nobuyuki 大阪大学, 大学院基礎工学研究科, 教授 (20281107)
KINOSHITA Masahiro 京都大学, エネルギー理工学研究所, 教授 (90195339)
IWAKI Mitsuhiro 理化学研究所, 生命システム研究センター, 上級研究員 (30432503)
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| Research Collaborator |
YAMAGUCHI Takaya 東北大学, 大学院工学研究科
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| Project Period (FY) |
2016-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | actomyosin / ATP energy / hydration free energy / driving force / hydration control / water entropy / polymorphism / Ca ion control / 筋収縮機構 / 化学力学変換 / アクトミオシン / 水の並進エントロピー / 分子モーター / 水和自由エネルギー / タンパク質の水和 / アクチンの構造多形 / タンパク質分子モーター / エントロピー駆動 / 水のエントロピー / 水和制御 |
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| Outline of Final Research Achievements |
In this study, we carried out an experiment to examine whether F-actin could be driven on a myosin-coated substrate by controlling the hydration state change of actomyosin complex without using ATP hydrolysis. Firstly, by CD spectroscopy, it was confirmed that the secondary and tertiary structures of F-actin were controlled by changing Mg(2+) and Ca(2+) concentrations using EGTA. Secondly, as the initial weak-binding state, acto-myosin (heavy meromyosin) complex with AMPPNP at relatively low ionic condition was formed. When the Ca2+ concentration was increased by using caged-Ca and irradiation of UV light, we observed ~60 nm displacement of a Q-dot attached on F-actin. Further experiments must be made to confirm this observation and to establish this new driving principle.
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Report
(2 results)
Research Products
(16 results)
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[Presentation] Strong Mg2+/Ca2+ Ion Dependence of Hydration of F-actin2016
Author(s)
Makoto Suzuki, Asato Imao, George Mogami, Ryotaro Chishima, Takahiro Watanabe, Takaya Yamaguchi, Nobuyuki Morimoto, Tetsuichi Wazawa
Organizer
Annual Meeting of Biophysical Society of Japan
Place of Presentation
Tsukuba International Congress center, Tsukuba, Japan
Year and Date
2016-11-25
Related Report
Int'l Joint Research
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