| Project/Area Number |
16K13931
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Physical chemistry
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| Research Institution | Osaka University |
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Principal Investigator |
KIM Sooyeon 大阪大学, 産業科学研究所, 特任助教(常勤) (50758886)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | 蛍光信号増幅 / 蛍光センサー / レージング / 顕微鏡 / 蛍光プローブ / 自己集合体 / 光増幅 / バイオセンシング / 細胞イメージング |
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| Outline of Final Research Achievements |
During this project, I developed (1) a sensor design that maximizes a fluorescence contrast inspired by whispering-gallery mode lasing (WGM), (2) fluorescence molecules that generate and detect reactive oxygen species, and (3) new microscopy technique based on WGM. First of all, fluorophore-aptamer-modified glass microspheres (cf. 1~38 μm) successfully detected a target protein, thrombin, in an excitation-power dependent manner (29-fold enhanced at 3400 μW). Next, we newly developed BODIPY and anthracene dyads that generate and detect reactive oxygen species. Based on these findings, various important insights for developing 'lasing microscopy' to visualize intracellular excited states have been obtained.
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