| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
Transient absorption (TA) spectroscopy was performed on the solution of photosystem II reaction center (PSII) in collaboration with a group in Okayama University, and the paper was published (Yoshida, Y., Yoshida, et al., J. Am. Chem. Soc., 138, (2016) 11599-11605). Subsequently, preliminary experiment on the microcrystal of PSII with femtosecond time-resolved TA micro-spectroscopy were conducted in a joint research with a group in Kwansei Gakuin University. PSII microcrystal was prepared by an oil-batch sandwich crystallization method, and the TA measurement was performed by selecting a thin microcrystal with a diameter of about 10 micrometers. For the measurement of coherent micro-spectroscopy by degenerate four-wave mixing, an inverted optical microscope was placed on a vibration isolation table and introduction of laser pulses from a non-collinear optical parametric amplifier (NOPA) excited with a regeneratively amplified Ti: sapphire laser is performed.
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