development of multi-wavelength periodic illumination technique for super-resolution microscopy

Research Project

Project/Area Number	16K14020
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Analytical chemistry
Research Institution	Osaka University
Principal Investigator	Yoshiki Nakata 大阪大学, レーザーエネルギー学研究センター, 准教授 (70291523)
Research Collaborator	SAITO Tetsuya 大阪大学, 大学院工学研究科
Project Period (FY)	2016-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000) Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords	超解像顕微鏡 / STED顕微鏡 / 構造化照明顕微鏡 / 干渉 / 位相 / 励起 / 脱励起 / レーザー / 超精密計測 / 応用光学・量子光工学 / 共焦点顕微鏡 / ナノバイオ / 分析科学
Outline of Final Research Achievements	In this experiment, the base technologies to combine STED super-resolution microscope and SIM microscope were developed. First, co-axial laser (λ=405nm & 488nm) was composed, then interference patterns at these wavelength with the same period Λ=126.0 micron was formed by a diffractive optical element and a Fourier optics. Second, the relative position of these interference patterns were controlled by means of wavelength dispersion of a glass plane. Third, double stage Fourier transfer systems was composed to avoid stray light. Then, interference patterns and images of fluorescent particles were imaged by the microscope with structured illumination.