| Project/Area Number |
16K14020
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Analytical chemistry
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| Research Institution | Osaka University |
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Principal Investigator |
Yoshiki Nakata 大阪大学, レーザーエネルギー学研究センター, 准教授 (70291523)
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| Research Collaborator |
SAITO Tetsuya 大阪大学, 大学院工学研究科
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 超解像顕微鏡 / STED顕微鏡 / 構造化照明顕微鏡 / 干渉 / 位相 / 励起 / 脱励起 / レーザー / 超精密計測 / 応用光学・量子光工学 / 共焦点顕微鏡 / ナノバイオ / 分析科学 |
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| Outline of Final Research Achievements |
In this experiment, the base technologies to combine STED super-resolution microscope and SIM microscope were developed. First, co-axial laser (λ=405nm & 488nm) was composed, then interference patterns at these wavelength with the same period Λ=126.0 micron was formed by a diffractive optical element and a Fourier optics. Second, the relative position of these interference patterns were controlled by means of wavelength dispersion of a glass plane. Third, double stage Fourier transfer systems was composed to avoid stray light. Then, interference patterns and images of fluorescent particles were imaged by the microscope with structured illumination.
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