| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
An adenosine triphosphate (ATP) is considered to a most important molecule as a molecular unit of currency in biological studies on energy metabolism. Our study is aimed at establishing a method that allows quantitative analysis of intracellular ATP level at targeted place at single cellular resolution. We generated a genetically encoded fluorescent sensor which comprises a green fluorescent protein variant and the part of the bacterial FoF1-ATP synthase. It exhibited the change in fluorescence as well as fluorescent lifetime in response to the change in ATP concentration. Thanks to this unique property, we were able to obtain the spatio-temporal information regarding ATP dynamics and analyse its concentration quantitatively at the same time at the same cell. More specifically, we succeeded to observe the difference of intra/sub-cellular ATP level between different cell types and organelles.
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