Development of optogenetics tools regulated by light with longer wavelength

• Project/Area Number: 16K14030
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Bio-related chemistry
• Research Institution: Osaka University (2017); University of Fukui (2016)
• Principal Investigator: Hattori Mitsuru 大阪大学, 産業科学研究所, 特任助教(常勤) (20589858)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 光遺伝学 / 蛍光観察 / 蛍光タンパク質 / 光応答タンパク質 / 光刺激 / オプトジェネティクス / 光制御 / 蛍光 / タンパク質

Outline of Final Research Achievements

We tried to establish a new optogenetics tool to regulate a protein in living cells by light with longer wavelength. For the purpose, near-field light from fluorescent molecules or photo-reactive proteins regulated by longer wavelength light were used.
In the method with photo-reactive proteins, we considered with a regulation of protein function by light. Ion-pump protein on cell membrane was selected, which was regulated by 580 nm light. The relationship between photo-reaction and variation of cell function were investigated with chemiluminescent protein sensor. In the result, cell phenomenon reacted with photo-irradiation were confirmed. Finally, we identified the possibility of photo-reactive proteins with the optical function with longer wavelength light.

Research Products

• [Journal Article] A genetic screen to discover SUMOylated proteins in living mammalian cells (2017)
  • Author(s): Komiya M, Ito A, Endo M, Hiruma D, Hattori M, Saitoh H, Yoshida M, Ozawa T.
  • Journal Title: Sci. Rep. Volume: 7 Issue: 1 Pages: 17443-17443
  • DOI: 10.1038/s41598-017-17450-7
  • Peer Reviewed / Open Access
• [Journal Article] Spatiotemporal analysis with a genetically encoded fluorescent RNA probe reveals TERRA function around telomeres. (2016)
  • Author(s): Yamada, T., Yoshimura, H., Shimada, R., Hattori, M., Eguchi, T., Fujiwara, K., Kusumi, A. and Ozawa, T.
  • Journal Title: Sci. Rep. Volume: 6 Issue: 1 Pages: 38910-38910
  • DOI: 10.1038/srep38910
  • NAID: 120005997833
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A Bioluminescent Indicator for Highly Sensitive Analysis of Estrogenic Activity in a Cell-based Format. (2016)
  • Author(s): Takenochi, O., Kanno, A., Takakura, H., Hattori, M. and Ozawa, T.
  • Journal Title: Bioconju.Chem. Volume: 7 Issue: 11 Pages: 2689-2694
  • DOI: 10.1021/acs.bioconjchem.6b00466
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Confocal Bioluminescence Imaging for Living Tissues with a Caged Substrate of Luciferin. (2016)
  • Author(s): Hattori, M., Kawamura, G., Kojima, R., Kamiya, M., Urano, Y. and Ozawa, T.
  • Journal Title: Anal. Chem. Volume: 88(12) Issue: 12 Pages: 6231-6238
  • DOI: 10.1021/acs.analchem.5b04142
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] ルシフェラーゼ再構成を基盤としたGPCRシグナルの発光定量検出法 (2017)
  • Author(s): 服部満
  • Organizer: 日本薬学会第137年会
  • Place of Presentation: 東北大学川内キャンパス (宮城県仙台市)
  • Year and Date: 2017-03-25
  • Invited
• [Presentation] Development a monitoring system of N-cadherin dependent neuronal adhesion (2017)
  • Author(s): Mitsuru Hattori, Tomoki Matsuda, Takeaki Ozawa, Takeharu Nagai
  • Organizer: Scientific research innovative areas, area-meeting
• [Presentation] 共焦点発光イメージングを利用した生細胞観察法の開発 (2016)
  • Author(s): 服部満, 河村玄気, 小嶋良輔, 神谷真子, 浦野泰照, 小澤岳昌
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜 (神奈川県横浜市)
  • Year and Date: 2016-11-30
• [Book] 先端計測 (日本化学会編) (2016)
  • Author(s): 服部満, 小澤岳昌 (分著)
  • Total Pages: 188
  • Publisher: 化学同人