| Project/Area Number |
16K14030
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bio-related chemistry
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| Research Institution | Osaka University (2017)
University of Fukui (2016) |
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Principal Investigator |
Hattori Mitsuru 大阪大学, 産業科学研究所, 特任助教(常勤) (20589858)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 光遺伝学 / 蛍光観察 / 蛍光タンパク質 / 光応答タンパク質 / 光刺激 / オプトジェネティクス / 光制御 / 蛍光 / タンパク質 |
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| Outline of Final Research Achievements |
We tried to establish a new optogenetics tool to regulate a protein in living cells by light with longer wavelength. For the purpose, near-field light from fluorescent molecules or photo-reactive proteins regulated by longer wavelength light were used.
In the method with photo-reactive proteins, we considered with a regulation of protein function by light. Ion-pump protein on cell membrane was selected, which was regulated by 580 nm light. The relationship between photo-reaction and variation of cell function were investigated with chemiluminescent protein sensor. In the result, cell phenomenon reacted with photo-irradiation were confirmed. Finally, we identified the possibility of photo-reactive proteins with the optical function with longer wavelength light.
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