Development of the system to change enzymatic functions without mutational approaches by using molecular crowding
| Project/Area Number |
16K14041
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bio-related chemistry
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| Research Institution | Konan University |
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Principal Investigator |
SUGIMOTO Naoki 甲南大学, 先端生命工学研究所, 教授 (60206430)
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| Co-Investigator(Kenkyū-buntansha) |
高橋 俊太郎 甲南大学, 先端生命工学研究所, 講師 (40456257)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 分子クラウディング / 核酸 / 酵素 / 複製 / 転写 / RNA / DNA / ポリメラーゼ / ポリエチレングリコール / 分子進化 |
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| Outline of Final Research Achievements |
We studied the effect of molecular crowding on DNA or RNA polymerization reactions and found that changes in molecular crowding caused the quantity and quality of polymerized products. These phenomenon were the process of gene regulations without protein. Thus, the structure of template nucleic acids played a role for gene expression in the RNA world where there was no protein. Furthermore, we identified that the regulation system is utilized in the modern cells by analysis of living cells. Therefore, in the process of transition from RNA world to DNA world, the gene regulation system by the structure of the template was took over form RNA to DNA.
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Report
(3 results)
Research Products
(22 results)
