Molecularly targeted photodynamic therapy targeting cancer related mRNA G-quadruplex
Project/Area Number |
16K14042
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Bio-related chemistry
|
Research Institution | Konan University |
Principal Investigator |
MIYOSHI Daisuke 甲南大学, フロンティアサイエンス学部, 教授 (50388758)
|
Co-Investigator(Kenkyū-buntansha) |
川内 敬子 甲南大学, フロンティアサイエンス学部, 講師 (40434138)
|
Project Period (FY) |
2016-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | 四重らせん構造 / RNA / NRAS / がん / 光線力学療法 / 分子標的型 / フタロシアニン / mRNA / リガンド / RAS / 活性酸素 / 分子標的 |
Outline of Final Research Achievements |
Ras signaling pathways contributes to aggressive phenotypes of cancer cells. The Ras-targeted therapies for cancer therefore has been recognized to be absolutely effective. On the other hand, it is well known that drug developments targeting Ras is very difficult due to structural feature of Ras protein. In this project, we found that expression of N-Ras was able to be controlled by photo-irradiation with an anionic phthalocyanine, ZnAPC, targeting NRAS mRNA, which forms a G-quadruplex at its 5' UTR region. It was shown that ZnAPC bound G-quadruplex-forming oligonucleotide derived from NRAS mRNA and cleaved it upon photo-irradiation. Consistent with these results, the combination of photo-irradiation and ZnAPC decreased N-Ras expression and drastically reduced cell viability of cancer cells, MCF-7 cells. These results demonstrate that ZnAPC is prominent for a molecular-targeted photodynamic cancer therapy.
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Report
(3 results)
Research Products
(46 results)
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[Journal Article] Unexpected Position-Dependent Effects of Ribose G-Quartets in G-Quadruplexes2017
Author(s)
Zhou Jun、Amrane Samir、Rosu Frederic、Salgado Gilmar F.、Bian Yunqiang、Tateishi-Karimata Hisae、Largy Eric、Korkut Dursun Nizam、Bourdoncle Anne、Miyoshi Daisuke、Zhang Jian、Ju Huangxian、Wang Wei、Sugimoto Naoki、Gabelica Valerie、Mergny Jean-Louis
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Journal Title
Journal of the American Chemical Society
Volume: 139
Issue: 23
Pages: 7768-7779
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] DNA G-wire formation using an artificial peptide is controlled by protease activity2017
Author(s)
Usui, K., Okada, A., Sakashita, S., Shimooka, M., Tsuruoka, T., Nakano, S., Miyoshi, D., Mashima, T., Katahira, M., Hamada, Y.
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Journal Title
Molecules
Volume: 22
Issue: 11
Pages: 1991-1991
DOI
NAID
Related Report
Peer Reviewed / Open Access
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