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Development of cross-reactive molecule libraries for generating information on antibody misfolding

Research Project

Project/Area Number 16K14043
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Bio-related chemistry
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

Shunsuke Tomita  国立研究開発法人産業技術総合研究所, バイオメディカル研究部門, 研究員 (50726817)

Co-Investigator(Kenkyū-buntansha) 白木 賢太郎  筑波大学, 数理物質系, 教授 (90334797)
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords抗体 / バイオセンサー / 酸化グラフェン / DNA / 多変量解析 / 高分子 / 機械学習 / バイオテクノロジー
Outline of Final Research Achievements

In recent years, antibodies have been widely used for medical purposes, but there is a problem that antibodies tend to degrade during purification, transportation and storage processes. Therefore, when designing therapeutic antibodies, it is necessary to strictly determine the methods and conditions of antibody processing and storage in each process based on a combination of separation and spectroscopic analyses. In this project, a novel sensing method was developed to address these issues. By using cross-reactive complexes between fluorescent DNAs and nanosized-graphene oxides (nGOs), the information on antibody misfolding was transduced into response fingerprints, and multivariate analyses of these fingerprints allowed for rapid and facile identification of the degraded states of the antibodies.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (19 results)

All 2018 2017 2016

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 1 results,  Acknowledgement Compliant: 3 results) Presentation (11 results) (of which Int'l Joint Research: 1 results,  Invited: 5 results) Book (1 results)

  • [Journal Article] A Multi-Fluorescent DNA/Graphene Oxide Conjugate Sensor for Signature-Based Protein Discrimination2017

    • Author(s)
      Tomita Shunsuke、Ishihara Sayaka、Kurita Ryoji
    • Journal Title

      Sensors

      Volume: 17 Issue: 10 Pages: 2194-2194

    • DOI

      10.3390/s17102194

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] One-Step Identification of Antibody Degradation Pathways Using Fluorescence Signatures Generated by Cross-Reactive DNA-Based Arrays2017

    • Author(s)
      Tomita Shunsuke、Matsuda Ayumi、Nishinami Suguru、Kurita Ryoji、Shiraki Kentaro
    • Journal Title

      Analytical Chemistry

      Volume: 89 Issue: 15 Pages: 7818-7822

    • DOI

      10.1021/acs.analchem.7b01264

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Environment-Sensitive Turn-On Fluorescent Polyamino Acid: Fingerprinting Protein Populations with Post-Translational Modifications2017

    • Author(s)
      Tomita Shunsuke、Ishihara Sayaka、Kurita Ryoji
    • Journal Title

      ACS Applied Materials & Interfaces

      Volume: 9 Issue: 27 Pages: 22970-22976

    • DOI

      10.1021/acsami.7b05360

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Protein Sensing Based on Cross-reactive Optical Fingerprinting2017

    • Author(s)
      冨田 峻介、吉本 敬太郎、丹羽 修、栗田 僚二
    • Journal Title

      BUNSEKI KAGAKU

      Volume: 66 Issue: 1 Pages: 1-10

    • DOI

      10.2116/bunsekikagaku.66.1

    • NAID

      130005313292

    • ISSN
      0525-1931
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Small Spheroids of Adipose-Derived Stem Cells with Time-Dependent Enhancement of IL-8 and VEGF-A Secretion2016

    • Author(s)
      Furuhata, Y., Kikuchi, Y., Tomita, S., Yoshimoto, K.
    • Journal Title

      Genes to Cells

      Volume: 21(12) Issue: 12 Pages: 1380-1386

    • DOI

      10.1111/gtc.12448

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Artificial modification of an enzyme for construction of cross-reactive polyion complexes to fingerprint signatures of proteins and mammalian cells2016

    • Author(s)
      Shunsuke Tomita, Osamu Niwa, Ryoji Kurita
    • Journal Title

      Analytical Chemistry

      Volume: 88 Issue: 18 Pages: 9079-9086

    • DOI

      10.1021/acs.analchem.6b02010

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Noncovalent PEGylation-based enzyme switch in physiological saline conditions using quaternization polyamines2016

    • Author(s)
      Kengo Kuwada, Takaaki Kurinomaru, Shunsuke Tomita, Kentaro Shiraki
    • Journal Title

      Colloid and Polymer Science

      Volume: 294 Issue: 10 Pages: 1551-1556

    • DOI

      10.1007/s00396-016-3916-5

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] 冨田 峻介2018

    • Author(s)
      アレイ型タンパク質フィンガープリント技術を利用したバイオセンシング
    • Organizer
      第2回TIA-TLSKライフイノベーションWS
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 酵素 / 合成高分子からなる複合体ライブラリを用いたセクレトーム解析による非侵襲な老化診断法2017

    • Author(s)
      野本 弘揮、冨田 峻介、吉本 敬太郎
    • Organizer
      日本化学会 第97春季年会
    • Place of Presentation
      慶應義塾大学 日吉キャンパス(神奈川県)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] 交差反応性蛍光ポリアミノ酸によるタンパク質の特徴情報の出力 / Fingerprinting of protein characteristics using cross-reactive fluorescent polyamino acids2017

    • Author(s)
      冨田 峻介、石原 紗綾夏、栗田 僚二
    • Organizer
      日本化学会 第97春季年会
    • Place of Presentation
      慶應義塾大学 日吉キャンパス(神奈川県)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] タンパク質の特徴情報を出力できる高分子アレイを利用したバイオメトリクス技術2017

    • Author(s)
      冨田 峻介
    • Organizer
      JST新技術説明会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 機械学習を活用したタンパク質分析法の開発と細胞評価への応用2017

    • Author(s)
      冨田 峻介
    • Organizer
      日本分析化学会第66年会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 冨田 峻介2017

    • Author(s)
      非特異的な相互作用を活用する―タンパク質の特徴パターンを出力できる分子群による生体試料メトリクス
    • Organizer
      蛋白研セミナー:産業応用を志向するタンパク質溶液研究
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 非共有結合的 PEG 化における非特異的な結合の解明2016

    • Author(s)
      栗之丸 隆章 、桑田 賢吾 、冨田 峻介 、亀田 倫史 、白木 賢太郎
    • Organizer
      第31回 高分子学会関東支部茨城地区 若手の会交流会
    • Place of Presentation
      つくばセミナーハウス(茨城県)
    • Year and Date
      2016-11-10
    • Related Report
      2016 Research-status Report
  • [Presentation] タンパク質の微少な構造の差異情報を出力可能な高分子材料の開発2016

    • Author(s)
      冨田 峻介、石原 紗綾夏、栗田 僚二
    • Organizer
      日本分析化学会第65年会
    • Place of Presentation
      北海道大学工学部(北海道)
    • Year and Date
      2016-09-14
    • Related Report
      2016 Research-status Report
  • [Presentation] Libraries of block-copolymer/enzyme complexes for protein fingerprinting: Toward markerless and noninvasive stem cell evaluation2016

    • Author(s)
      Shunsuke Tomita, Keitaro Yoshimoto, Osamu Niwa, Ryoji Kurita
    • Organizer
      ISPAC2016
    • Place of Presentation
      Borneo Convention Centre Kuching, Kuching, (Malaysia)
    • Year and Date
      2016-08-15
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Rolling Circle Amplification(RCA)法を利用した5-メチルシトシンの増幅検出法2016

    • Author(s)
      吉田 岳史、冨田 峻介、 鈴木 博章、栗田 僚二
    • Organizer
      第76回分析化学討論会
    • Place of Presentation
      岐阜薬科大学・岐阜大学(岐阜県)
    • Year and Date
      2016-05-28
    • Related Report
      2016 Research-status Report
  • [Presentation] 分泌タンパク質組成を反映したフィンガープリントを出力する材料ライブラリ2016

    • Author(s)
      冨田 峻介、栗田 僚二、吉本 敬太郎
    • Organizer
      第4回バイオ関連化学シンポジウム若手フォーラム
    • Place of Presentation
      もてなしドーム(石川県)
    • Related Report
      2016 Research-status Report
  • [Book] Biomedical Engineering Challenges: A Chemical Engineering Insight2018

    • Author(s)
      Vincenzo Piemonte (Editor), Angelo Basile (Editor), Taichi Ito (Editor), Luigi Marrelli (Editor)
    • Total Pages
      256
    • Publisher
      John Wiley & Sons, Inc
    • ISBN
      9781119296010
    • Related Report
      2017 Annual Research Report

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Published: 2016-04-21   Modified: 2019-03-29  

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