Development of In-Cell Imaging Technique for Studying Molecular Dynamics in Living Cells
| Project/Area Number |
16K14132
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Production engineering/Processing studies
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| Research Institution | Toyohashi University of Technology |
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Principal Investigator |
SHIBATA TAKAYUKI 豊橋技術科学大学, 工学(系)研究科(研究院), 教授 (10235575)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 走査型プローブ顕微鏡 / チップ増強ラマン分光法 / 細胞機能解析 / 細胞操作 / バイオMEMS / チップ増強型ラマン分光法 |
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| Outline of Final Research Achievements |
With the aim of developing an in-cell imaging technique for studying the dynamic behavior of biological molecules inside cells with high spatial and temporal resolution, we developed a fabrication process for a flat rectangular fluidic microchannel embedded into a Si cantilever beam structure for the geometric optimization of a newly designed AFM probe we call a "bioprobe" that is integrated with a sharp-tipped hollow nanoneedle instead of a conventional solid tip. Moreover, we demonstrated the possibility of utilizing tip-enhanced Raman spectroscopy (TERS) for enabling quantitative study on molecular dynamic processes inside single living cells with the homemade Raman system.
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Report
(3 results)
Research Products
(8 results)
