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Measurement of bactericidal effects by reactive species for sterilization using plasma

Research Project

Project/Area Number 16K14208
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Power engineering/Power conversion/Electric machinery
Research InstitutionThe University of Tokyo

Principal Investigator

Ono Ryo  東京大学, 大学院新領域創成科学研究科, 准教授 (90323443)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordssterilization / discharge plasma / reactive species / vacuum ultraviolet / photolysis / plasma medicine / 放電プラズマ / 活性種 / 殺菌 / 真空紫外光 / 光解離反応 / OHラジカル
Outline of Final Research Achievements

In the sterilization using discharge plasma including plasma medicine and water treatment, reactive species such as OH radicals are considered to cause the sterilization. However, the bactericidal effects by those short-lived reactive species including OH radicals have not been directly measured. In this study, we quantitatively measured the bactericidal effects by OH radicals, ozone, and H2O2 on Bacillus subtilis and Escherichia coli using the method we have developed, which selectively produces specific types of reactive species using photolysis by vacuum ultraviolet light. Then, we compared the bactericidal effects by OH, ozone, and H2O2, and the bactericidal effects on the two types of bacteria. In addition, we measured the densities of reactive species produced by this method to verify the reliability of the simulation which calculates the densities of reactive species of this method.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (32 results)

All 2018 2017 2016

All Journal Article (6 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 6 results,  Acknowledgement Compliant: 5 results,  Open Access: 1 results) Presentation (26 results) (of which Int'l Joint Research: 13 results,  Invited: 8 results)

  • [Journal Article] Plasma-induced suppression of recurrent and re-inoculated melanoma tumors in mice2018

    • Author(s)
      Mizuno Kazue、Shirakawa Yuki、Sakamoto Tatsuya、Ishizaki Hiromu、Nishijima Yoshimi、Ono Ryo
    • Journal Title

      IEEE Transactions on Radiation and Plasma Medical Sciences

      Volume: 印刷中 Issue: 4 Pages: 353-359

    • DOI

      10.1109/trpms.2018.2809673

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Two-Dimensional Electron Density Measurement of Pulsed Positive Streamer Discharge in Atmospheric-Pressure Air2017

    • Author(s)
      Yuki Inada, Kaiho Aono, Ryo Ono, Akiko Kumada, Kunihiko Hidaka and Mitsuaki Maeyama
    • Journal Title

      Journal of Physics D: Applied Physics

      Volume: 50 Issue: 17 Pages: 174005-174005

    • DOI

      10.1088/1361-6463/aa65ee

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Measurement of singlet delta oxygen in an atmospheric-pressure helium-oxygen plasma jet2017

    • Author(s)
      Y. Inoue, R. Ono
    • Journal Title

      Journal of Physics D: Applied Physics

      Volume: 印刷中 Issue: 21 Pages: 214001-214001

    • DOI

      10.1088/1361-6463/aa6c53

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Anti-Tumor Immune Response Induced by Nanosecond Pulsed Streamer Discharge in Mice2017

    • Author(s)
      K. Mizuno, K. Yonetamari, Y. Shirakawa, T. Akiyama, R. Ono
    • Journal Title

      Journal of Physics D: Applied Physics

      Volume: 50 Issue: 12 Pages: 12LT01-12LT01

    • DOI

      10.1088/1361-6463/aa5dbb

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Measurement of singlet delta oxygen in an atmospheric-pressure helium-oxygen plasma jet2017

    • Author(s)
      Y. Inoue and R. Ono
    • Journal Title

      Journal of Physics D: Applied Physics

      Volume: 印刷中

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Inactivation of Bacillus Atrophaeus by OH Radicals2016

    • Author(s)
      R. Ono, K. Yonetamari, Y. Tokumitsu, S. Yonemori, H. Yasuda, A. Mizuno
    • Journal Title

      Journal of Physics D: Applied Physics

      Volume: 49 Issue: 30 Pages: 305401-305401

    • DOI

      10.1088/0022-3727/49/30/305401

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] ナノ秒パルスプラズマを用いたマウスメラノーマの抗腫瘍効果の検証と免疫応答の病理解析2018

    • Author(s)
      坂本 達哉, 石崎 啓, 水野 和恵, 西島 良美, 小野 亮
    • Organizer
      2018年度静電気学会春期講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 水面上パルスストリーマ放電におけるOHラジカルのLIF計測2018

    • Author(s)
      青野 海豊, 小野 亮
    • Organizer
      2018年度静電気学会春期講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 真空紫外光を用いたOHの殺菌効果の検証と定量化2017

    • Author(s)
      岩瀬 篤郎, 小野 亮
    • Organizer
      2017年度静電気学会春期講演会
    • Place of Presentation
      東京大学 (東京都、文京区)
    • Year and Date
      2017-03-07
    • Related Report
      2016 Research-status Report
  • [Presentation] VUV法による活性種の選択的生成とシミュレーションの検証2017

    • Author(s)
      川野邉 素, 小野 亮
    • Organizer
      2017年度静電気学会春期講演会
    • Place of Presentation
      東京大学 (東京都、文京区)
    • Year and Date
      2017-03-07
    • Related Report
      2016 Research-status Report
  • [Presentation] 大気圧He/O2プラズマジェットにおけるO2(a)密度その場計測2017

    • Author(s)
      井上 湧貴, 小野 亮
    • Organizer
      2017年度静電気学会春期講演会
    • Place of Presentation
      東京大学 (東京都、文京区)
    • Year and Date
      2017-03-07
    • Related Report
      2016 Research-status Report
  • [Presentation] Measurement of singlet delta oxygen in atmospheric-pressure helium-oxygen plasma jet2017

    • Author(s)
      Y. Inoue and R. Ono
    • Organizer
      International Conference on Plasma Medical Science Innovation 2017
    • Place of Presentation
      Nagoya University (Nagoya, Japan)
    • Year and Date
      2017-02-27
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Measurement of density and sterilization effect of specific reactive species with the aid of vacuum ultraviolet photolysis2017

    • Author(s)
      M. Kawanobe, A. Iwase, and R. Ono
    • Organizer
      International Conference on Plasma Medical Science Innovation 2017
    • Place of Presentation
      Nagoya University (Nagoya, Japan)
    • Year and Date
      2017-02-27
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Cancer cell treatment in vitro using various sources of reactive species2017

    • Author(s)
      T. Sakamoto, Y. Shirakawa, H. Ishizaki, and R. Ono
    • Organizer
      International Conference on Plasma Medical Science Innovation 2017
    • Place of Presentation
      Nagoya University (Nagoya, Japan)
    • Year and Date
      2017-02-27
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 大気圧プラズマの計測とモデリング2017

    • Author(s)
      小野 亮
    • Organizer
      日本学術振興会153委員会 プラズマスクール
    • Place of Presentation
      弘済会館 (東京都、千代田区)
    • Year and Date
      2017-02-23
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] Measurement of singlet delta oxygen in an atmospheric-pressure helium-oxygen plasma jet2017

    • Author(s)
      Y. Inoue and R. Ono
    • Organizer
      IOP Publishing Young Researchers' Meeting: Frontiers in Fundamental and Applied Physics
    • Place of Presentation
      The University of Tokyo (Tokyo, Japan)
    • Year and Date
      2017-02-21
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Measurement of singlet delta oxygen metastable in atmospheric pressure He/O2 plasma jet2017

    • Author(s)
      Y. Inoue and R. Ono
    • Organizer
      34th Symposium on Plasma Processing / The 29th Symposium on Plasma Science for Materials
    • Place of Presentation
      北海道大学 (北海道、札幌市)
    • Year and Date
      2017-01-16
    • Related Report
      2016 Research-status Report
  • [Presentation] Supply of reactive species onto liquid surface produced using vacuum ultraviolet photolysis and measurement of their effects on liquid treatment2017

    • Author(s)
      R. Ono, A. Iwase, and H. Ishizaki
    • Organizer
      The 10th EU-Japan Joint Symposium on Plasma Processing
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Shack-Hartmann sensor for two-dimensional electron density measurement over positive primary streamer propagating in atmospheric-pressure air2017

    • Author(s)
      Y. Inada, R. Ono, A. Kumada, K. Hidaka and M. Maeyama
    • Organizer
      23rd International Symposium on Plasma Chemistry
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Two-dimensional electron density distribution over positive primary streamer propagating in atmospheric-pressure air2017

    • Author(s)
      Y. Inada, R. Ono, A. Kumada, K. Hidaka and M. Maeyama
    • Organizer
      33th International Conference on Phenomena in Ionized Gases
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Measurement of propagation, radical densities, temperature, and electron density of streamer discharge2017

    • Author(s)
      R. Ono
    • Organizer
      6th EUCASS Aerospace Thematic Workshop, Fundamentals of Aerodynamic Flow and Combustion Control by Plasma
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 医療用大気圧プラズマ反応場におけるラジカル計測2017

    • Author(s)
      小野 亮
    • Organizer
      Plasma Conference 2017
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] パルスストリーマ放電下における気液界面でのOHラジカルの計測2017

    • Author(s)
      青野 海豊, 小野 亮
    • Organizer
      第41回静電気学会全国大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ナノ秒パルスストリーマ放電を用いたマウスメラノーマの肺転移抑制効果の検証2017

    • Author(s)
      坂本 達哉, 石崎 啓, 水野 和恵, 西島 良美, 小野 亮
    • Organizer
      第41回静電気学会全国大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] プラズマ計測の今後の展開 - プラズマのより深い理解に向けて2017

    • Author(s)
      小野 亮
    • Organizer
      プラズマ計測の今後の展開 - プラズマのより深い理解に向けて
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Immunotherapy of cancer using plasma and measurement of reactive species effective for plasma medicine2016

    • Author(s)
      R. Ono
    • Organizer
      26th Annual Meeting of The Materials Research Society of Japan
    • Place of Presentation
      Yokohama Port Opening Plaza (Kanagawa, Japan)
    • Year and Date
      2016-12-19
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] VUV法による活性種密度の計測とシミュレーション結果との比較2016

    • Author(s)
      川野邉 素, 岩瀬 篤郎, 小野 亮
    • Organizer
      第40回静電気学会全国大会
    • Place of Presentation
      群馬大学 (群馬県、前橋市)
    • Year and Date
      2016-09-29
    • Related Report
      2016 Research-status Report
  • [Presentation] 大気圧He/O2プラズマジェットにおけるO2(a)赤外発光分光計測2016

    • Author(s)
      井上 湧貴, 小野 亮
    • Organizer
      第40回静電気学会全国大会
    • Place of Presentation
      群馬大学 (群馬県、前橋市)
    • Year and Date
      2016-09-29
    • Related Report
      2016 Research-status Report
  • [Presentation] Measurement of singlet delta oxygen metastable in atmospheric pressure helium plasma jet2016

    • Author(s)
      R. Ono and Y. Inoue
    • Organizer
      21st International Conference on Gas Discharges and Their Applications
    • Place of Presentation
      Nagoya University (Nagoya, Japan)
    • Year and Date
      2016-09-11
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Measurement of the medical effect of specific reactive species with the aid of vacuum ultraviolet photolysis2016

    • Author(s)
      M. Kawanobe, K. Yonetamari, Y. Shirakawa, and R. Ono
    • Organizer
      21st International Conference on Gas Discharges and Their Applications
    • Place of Presentation
      Nagoya University (Nagoya, Japan)
    • Year and Date
      2016-09-11
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Measurement of reactive species in atmospheric pressure plasmas2016

    • Author(s)
      R. Ono
    • Organizer
      2016 Plasma Processing Science Gordon Research Conference
    • Place of Presentation
      New Hampshire (USA)
    • Year and Date
      2016-07-24
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Optical diagnostics and selective supply of reactive species for plasma medicine2016

    • Author(s)
      R. Ono
    • Organizer
      18th International Congress on Plasma Physics
    • Place of Presentation
      Kaohsiung (Taiwan)
    • Year and Date
      2016-06-27
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited

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Published: 2016-04-21   Modified: 2019-03-29  

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