Fabrication and development of biosensor to detect aggregation of amyloid-beta oligomer
| Project/Area Number |
16K14254
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Electron device/Electronic equipment
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| Research Institution | Kyoto Institute of Technology |
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Principal Investigator |
Noda Minoru 京都工芸繊維大学, 電気電子工学系, 教授 (20294168)
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| Co-Investigator(Kenkyū-buntansha) |
徳田 隆彦 京都府立医科大学, 医学(系)研究科(研究院), 教授 (80242692)
島内 寿徳 岡山大学, 環境生命科学研究科, 准教授 (10335383)
寒川 雅之 新潟大学, 自然科学系, 准教授 (70403128)
山下 馨 京都工芸繊維大学, 電気電子工学系, 准教授 (40263230)
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| Project Period (FY) |
2016-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | バイオセンサー / Ａβ / オリゴマー / 動態 / Aβ / バイオセンサ－ |
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| Outline of Final Research Achievements |
Liposome biosensors are developed to detect dynamics of aggregation and fibrillization of amyloid-beta oligomer. Those sensors are expected to realize early and simple diagnosis and evaluation of progress in pathological change of Alzheimer Disease that expresses aggregation and accumulation of abnormal protein. In this research, both sensor device and biosensing phospholipid of liposome are investigated and developed as follows: I. Construction of the sensor device, II. Improvement in sensitivity of the sensor, and III. Comparison with ELISA result. A micro-TAS chip comprising of liposome cantilever sensor and microfluidic channels was fabricated. Consequently, dynamic aggregation and fibrillization of amyloid-beta related to its oligomer was selectively detected in human serum. Also, a high sensitivity was estimated that can detect less than 1 nM by excellent selection of phospholipid species, techniques of low-noise electronic circuit and precise temperature control of substrate.
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Report
(2 results)
Research Products
(36 results)
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[Journal Article] Monoclonal antibody with conformational specificity for a toxic conformer of amyloid β42 and its application toward the Alzheimer's disease diagnosis2016
Author(s)
Kazuma Murakami, Maki Tokuda, Takashi Suzuki, Yumi Irie, Mizuho Hanaki, Naotaka Izuo, Yoko Monobe, Ken-ichi Akagi, Ryotaro Ishii, Harutsugu Tatebe, Takahiko Tokuda, Masahiro Maeda, Toshiaki Kume, Takahiko Shimizu, Kazuhiro Irie
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Journal Title
Sci. Rep.
Volume: 6
Issue: 1
Pages: 29038-29038
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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