Development of cell-integrated micro-photonic device

• Project/Area Number: 16K14256
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Electron device/Electronic equipment
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: Ohta Jun 奈良先端科学技術大学院大学, 物質創成科学研究科, 教授 (80304161)
• Co-Investigator(Renkei-kenkyūsha): TOKUDA Takashi 奈良先端科学技術大学院大学, 物質創成科学研究科, 准教授 (50314539) ; SASAGWA Kiyotaka 奈良先端科学技術大学院大学, 物質創成科学研究科, 助教 (50392725) ; NODA Toshihiko 奈良先端科学技術大学院大学, 物質創成科学研究科, 助教 (20464159) ; TAKEHARA Hiroaki 奈良先端科学技術大学院大学, 研究推進機構, 特任助教 (60723088)
• Project Period (FY): 2016-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2016: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
• Keywords: 集積回路 / 細胞 / イメージセンサ / 蛍光 / フォトニックデバイス / 細胞センサ / LSI / バイオデバイス

Outline of Final Research Achievements

We have succeeded to culture GFP expressed HeLa cells on our micro-photonic device. An adhesive layer has been developed for this purpose. The absorption filter to detect green fluorescence from GFP and block blue light from an excitation light source have already been developed in our lab and can be applied to this experiment. The whole device is put into a PBS solution.
Next, we dropped a histamine solution onto the device. We have successfully demonstrated to obtain fluorescent image from the cells when a histamine solution is dropped. This clearly shows our device can be used as a cell-sensor to detect a chemical substance through fluorescence.

Research Products

• [Presentation] CMOS イメージセンサを用いた培養細胞のレンズレスオンチップ蛍光計測システム (2017)
  • Author(s): 中元 健太, 大澤 和嵩, 春田 牧人, 野田 俊彦, 笹川 清隆, 徳田 崇, 太田 淳
  • Organizer: 第64回応用物理学会春季学術講演会
  • Place of Presentation: パシフィコ横浜 神川県 横浜市
  • Year and Date: 2017-03-14
• [Presentation] Fabrication of a Prototype Dual Filter CMOS Image Sensor for FRET Imaging (2017)
  • Author(s): Hee Wan Shen,Haruta Makito,Noda Toshihiko,Sasagawa Kiyotaka,Tokuda Takashi,Ohta Jun
  • Organizer: 第64回応用物理学会春季学術講演会
  • Place of Presentation: パシフィコ横浜 神川県 横浜市
  • Year and Date: 2017-03-14
• [Presentation] 生体内埋植 CMOS デバイスを用いた生体機能の光による計測と操作 (2017)
  • Author(s): 太田 淳
  • Organizer: 日本学術振興会先端ナノデバイス・材料テクノロジー第151委員会
  • Place of Presentation: LiSE 川崎生命科学・環境研究センター 神奈川県川崎市
  • Year and Date: 2017-03-02
  • Invited
• [Presentation] Implantable optoelectronic devices for controlling and measuring biological functions (2017)
  • Author(s): Jun Ohta
  • Organizer: The 8th MEI3 Center International Symposium
  • Place of Presentation: "Center of Medical Innovation and Translational Research Building, Suita, Osaka
  • Year and Date: 2017-03-01
  • Int'l Joint Research / Invited
• [Presentation] An implantable imaging device for observing the recovery process from cerebrovascular disease in small animal brains (2016)
  • Author(s): Makito Haruta, Yoshinori Sunaga, Takahiro Yamaguchi, Toshihiko Noda, Kiyotaka Sasagawa, Takashi Tokuda, Jun Ohta
  • Organizer: 2016 GLOBAL RESEARCH EFFORTS ON ENERGY AND NANOMATERIALS
  • Place of Presentation: GIS TAIPEI TECH Convention Center,Taiwan
  • Year and Date: 2016-12-24
  • Int'l Joint Research / Invited
• [Presentation] A portable cell imaging system with dual imaging functionality (2016)
  • Author(s): Makito Haruta, Anek Wuthayavanich, Kazutaka Osawa, Toshihiko Noda, Kiyotaka Sasagawa, Takashi Tokuda, Jun Ohta
  • Organizer: 5th International Conference on BioSensors, BioElectronics, BioMedical Devices, BioMEMS/NEMS & Applications 2016 (Bio4Apps2016)
  • Place of Presentation: Griffith University, QLD, Australia
  • Year and Date: 2016-12-14
  • Int'l Joint Research / Invited
• [Presentation] 光電子デバイス技術による生体体機能の計測と操作 (2016)
  • Author(s): 太田 淳
  • Organizer: 計測自動制御学会 ライフエンジニアリング部門シンポジウム2016
  • Place of Presentation: 大阪国際交流センター 大阪府大阪市
  • Year and Date: 2016-11-04
  • Invited
• [Presentation] Optical-based Technologies for Brain Imaging and Manipulation (2016)
  • Author(s): Jun Ohta
  • Organizer: The 2016 IEEE International Conference on Systems, Man, and Cybernetics (SMC 2016)
  • Place of Presentation: Hotel Intercontinental Budapest, Budapest, Hungary
  • Year and Date: 2016-10-12
  • Int'l Joint Research / Invited
• [Presentation] バイオ医療分野における半導体デバイスの役割と今後の展開 (2016)
  • Author(s): 太田 淳
  • Organizer: DAシンポジウム2016
  • Place of Presentation: 山代温泉 ゆのくに天祥 石川県加賀市
  • Year and Date: 2016-09-16
  • Invited
• [Presentation] 細胞外因子に対する細胞応答のオンチップ蛍光計測 (2016)
  • Author(s): 大澤 和嵩，竹原 宏明，野田 俊彦，笹川 清隆，徳田 崇，太田 淳
  • Organizer: 第77回応用物理学会秋季学術講演会
  • Place of Presentation: 朱鷺メッセ 新潟県新潟市
  • Year and Date: 2016-09-16
• [Presentation] 埋植型光電子デバイスのバイオ医療への応用 (2016)
  • Author(s): 太田 淳
  • Organizer: 第77回応用物理学会秋季学術講演会
  • Place of Presentation: 朱鷺メッセ 新潟県新潟市
  • Year and Date: 2016-09-15
  • Invited
• [Presentation] CMOS-Based Opto-Electronic Neural Interface Devices for Optogenetics (2016)
  • Author(s): Tokuda Takashi,Noguchi Satoki,Iwasaki Satoru,Takehara Hiroaki,Noda Toshihiko,Sasagawa Kiyotaka, Ohta Jun
  • Organizer: The 38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC’16)
  • Place of Presentation: Walt Disney World Resort, Orlando, FL, USA
  • Year and Date: 2016-08-20
  • Int'l Joint Research / Invited
• [Presentation] Micro-Optoelecronic Devices for Biomedical Applications (2016)
  • Author(s): Jun Ohta
  • Organizer: 2016 International Conference on Optical MEMS and Nanophotonics
  • Place of Presentation: National University of Singapore, Singapore, Singapore
  • Year and Date: 2016-08-02
  • Int'l Joint Research / Invited
• [Presentation] Imaging Technology for Implantable Biomedical Applications (2016)
  • Author(s): Jun Ohta
  • Organizer: International Symposium on Biomedical Engineering 2016
  • Place of Presentation: Margo Hotel, Depok Java, Indonesia
  • Year and Date: 2016-05-30
  • Int'l Joint Research / Invited