PCR-free DNA diagnosis using dielectrophoresis
| Project/Area Number |
16K14278
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Measurement engineering
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| Research Institution | Kyushu University |
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Principal Investigator |
Suehiro Junya 九州大学, システム情報科学研究院, 教授 (70206382)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKANO Michihiko 九州大学, 大学院システム情報科学研究院, 准教授 (00447856)
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| Research Collaborator |
DING Zhenhao 九州大学, 大学院システム情報科学府
IDA Kenichi 九州大学, 大学院システム情報科学府
MATSUDA Kenya 九州大学, 大学院システム情報科学府
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 計測システム / 誘電泳動 / DNA診断 / MEMS / PCR / 遺伝子 / マイクロ流体デバイス / DNA / マイクロデバイス |
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| Outline of Final Research Achievements |
A novel micro fluidic device was proposed in order to detect alteration of negative dielectrophoretic force acting on microbeads decorated by DNA molecules. It was demonstrated that the microbead trajectory was apparently changed when the microbead surface was chemically decorated with DNA molecules more than 1000 units. By using fluorescence microbeads and an optical detection system, which could count the number of microbeads, the trajectory change was successfully quantified. This optical detection method realized detection of microbead whose surface was chemically decorated with DNA molecules more than 100 units. The DNA detection sensitivity is 100 times higher than the conventional method, which also utilizes dielectrophoresis of DNA-decorated microbeads.
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Report
(3 results)
Research Products
(8 results)
