| Project/Area Number |
16K14333
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Civil and environmental engineering
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| Research Institution | Tohoku Gakuin University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | ヒ素 / 検出システム / バイオセンサー / ヒ素耐性遺伝子群 / ヒ素濃度測定 / レポーター遺伝子 / ArsR / ヒ酸 / 亜ヒ酸 / Rhodococcus / 転写制御 / 測定 |
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| Outline of Final Research Achievements |
An Arsenic detection system using bacteria and their arsenic resistant system was developed. A reporter plasmid in which the arsR gene and the promoter region of arsenic resistant genes including ArsR-binding site were inserted upstream of the luciferase gene, was constructed. The ArsR represses the transcription of the arsenic resistant genes and de-represses it in the presence of arsenite. It was shown that the resultant transformant can detect both arsenate and arsenite. By using a mutant lacking arsenite efflux pump genes, the sensitivity toward arsenic was enhanced. By adding the phosphate buffer during incubation, the strain can detect only arsenite.
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| Academic Significance and Societal Importance of the Research Achievements |
サンプルと混合してインキュベートし発光量を測定するだけで、環境基準値以下の濃度のサンプルでも亜ヒ酸を検出可能な系を構築することができた。また、既知のヒ酸還元酵素遺伝子以外にもヒ酸還元に関与している遺伝子の存在が示唆されるなど、細菌とヒ素の関わりに関する新たな知見を得ることができた。
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