Synthesis of organic nanocrystals with the function of molecular recognition via material-binding proteins

• Project/Area Number: 16K14483
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biofunction/Bioprocess
• Research Institution: Tohoku University
• Principal Investigator: Umetsu Mitsuo 東北大学, 工学研究科, 教授 (70333846)
• Co-Investigator(Renkei-kenkyūsha): MATSUI JUN 山形大学, 理学部, 准教授 (50361184)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 生体分子 / 生体機能利用 / ナノ材料

Outline of Final Research Achievements

Nano-sized organic crystal particles are applicable for bioimaging. However, the particles are too hydrophobic to be dispersed in water, and the binding of antibody on the surface is difficult. In this study, we generated an interface molecule which can make a linkage between organic material surface and antibody, and we proposed a new method for immobilize antibodies on the surface of organic materials in one pot.

Academic Significance and Societal Importance of the Research Achievements

有機蛍光分子の結晶粒子は、分子の配向構造によっては輝度・寿命が飛躍的に向上し、発光波長も変化させることができるが、蛍光分子の基本骨格は疎水性であるため作製したナノ結晶は水溶液中では分散しにくく、結晶表面に抗体などの分子標的機能を付与させる汎用的方法がない。本研究の成果より、特定の有機材料表面に吸着できるペプチドやタンパク質を取得することによって、有機ナノ素材の物性を変化させることができ、有機ナノ材および有機界面のバイオイメージングやセンシングへの展開範囲を拡張させることができた。

Research Products

• [Journal Article] Use of a Phage-Display Method to Identify Peptides that Bind to a Tin Oxide Nanosheets (2018)
  • Author(s): Nakazawa Hikaru、Seta Yasuko、Hirose Tatsuya、Masuda Yoshitake、Umetsu Mitsuo
  • Journal Title: Protein & Peptide Letters Volume: 25 Issue: 1 Pages: 68-75
  • DOI: 10.2174/0929866525666171206114429
  • Peer Reviewed
• [Journal Article] Impact in stability during sequential CDR grafting to construct camelid VHH antibodies against zinc oxide and gold (2018)
  • Author(s): Saito Ryota、Saito Yutaro、Nakazawa Hikaru、Hattori Takamitsu、Kumagai Izumi、Umetsu Mitsuo、Makabe Koki
  • Journal Title: The Journal of Biochemistry Volume: 164 Issue: 1 Pages: 21-25
  • DOI: 10.1093/jb/mvy016
  • NAID: 40021625279
  • Peer Reviewed
• [Journal Article] Structural considerations for functional anti-EGFR × anti-CD3 bispecific diabodies in light of domain order and binding affinity (2018)
  • Author(s): 1.Asano R., Nagai K., Makabe K., Takahashi K., Kumagai T., Kawaguchi H., Ogata H., Arai K., Umetsu M., Kumagai I.
  • Journal Title: Oncotarget Volume: 9 Issue: 17 Pages: 13884-13893
  • DOI: 10.18632/oncotarget.24490
  • Peer Reviewed / Open Access
• [Journal Article] Photosynthesis of colloidal Ag-AgCl nanoparticles mediated by Tilia sp. leachate, evaluation of their behaviour in liquid phase and catalytic properties (2018)
  • Author(s): Zuzana Konvickova, Veronika sova, Marek Kolencik, Teppei Niide, Gabriela Kratosova, Mitsuo Umetsu, and Jana Seidlerova
  • Journal Title: Colloid and Polymer Science Volume: 296 Issue: 4 Pages: 677-687
  • DOI: 10.1007/s00396-018-4290-2
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] High-throughput cytotoxicity and antigen-binding assay for screening small bispecific antibodies without purification (2018)
  • Author(s): Sugiyama Aruto、Umetsu Mitsuo、Nakazawa Hikaru、Niide Teppei、Asano Ryutaro、Hattori Takamitsu、Kumagai Izumi
  • Journal Title: JBB Volume: - Issue: 2 Pages: 31237-31239
  • DOI: 10.1016/j.jbiosc.2018.02.007
  • NAID: 40021645320
  • Peer Reviewed
• [Journal Article] A semi high-throughput method for screening small bispecific antibodies with high cytotoxicity (2017)
  • Author(s): Sugiyama Aruto、Umetsu Mitsuo、Nakazawa Hikaru、Niide Teppei、Onodera Tomoko、Hosokawa Katsuhiro、Hattori Shuhei、Asano Ryutaro、Kumagai Izumi
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 2862-2873
  • DOI: 10.1038/s41598-017-03101-4
  • Peer Reviewed / Open Access
• [Journal Article] Generation of camelid VHH bispecific constructs via in-cell intein-mediated protein trans-splicing (2017)
  • Author(s): Yuki Shibuya, Natsuki Haga, Ryutaro Asano, Hikaru Nakazawa, Takamitsu Hattori, Daisuke Takeda Aruto Sugiyama, Reiko Kurotani, Izumi Kumagai, Mitsuo Umetsu, Koki Makabe
  • Journal Title: Protein Engineering, Design & Selection Volume: 30 Pages: 15-21
  • DOI: 10.1093/protein/gzw057
  • Peer Reviewed
• [Journal Article] Isomorphic coalescence of aster cores formed in vitro from microtubules and kinesin motors (2016)
  • Author(s): Kyongwan Kim, Aurelien Sicora; Hikaru Nakazawa, Mitsuo Umetsu, Wonmuk Hwang, Winfried Teizer
  • Journal Title: Physical Biology Volume: 13 Issue: 5 Pages: 1-18
  • DOI: 10.1088/1478-3975/13/5/056002
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Smart bio-design for hybrid protein molecules (2017)
  • Author(s): Mitsuo Umetsu
  • Organizer: UNIST seminar
  • Int'l Joint Research / Invited
• [Presentation] Nanobiotechnology from material-binding biomolecules for nanomaterial engineering (2017)
  • Author(s): Mitsuo Umetsu
  • Organizer: International Workshop on Advanced Smart Materials and Engineering for Nano- and BioTechnologies
  • Int'l Joint Research / Invited
• [Presentation] ナノとバイオをつなぐインターフェイスプロテイン (2017)
  • Author(s): 梅津 光央
  • Organizer: 第１７４委員会第５９回研究会
  • Invited
• [Presentation] 機械学習へ向けた迅速な変異体群の作製と機能評価プロセスの開発 (2017)
  • Author(s): 及川未早来、中澤光、二井手哲平、亀田倫史、斎藤裕、津田浩二、梅津光央
  • Organizer: バイオ工学シンポジウム
• [Presentation] 抗体に代わる分子標的バイオ医薬フォーマットライブラリーの構築 (2017)
  • Author(s): 伊藤智之、二井手哲平、中澤光、西羽美、亀田倫史、梅津光央
  • Organizer: バイオ工学シンポジウム
• [Presentation] 低分子医薬の欠点を克服する医薬ペプチドの開発 (2017)
  • Author(s): 高橋拓人、二井手哲平、中澤光、今中洋行、梅津光央
  • Organizer: バイオ工学シンポジウム
• [Presentation] Easy design of nano-oriented interface molecule on material chip: application of using substrate material binding protein for biosensing (2017)
  • Author(s): Tatsuki Miyaki , HikaruNakazawa , Takuma Sujino , and Mitsuo Umetsu
  • Organizer: The 13rd The Asian Congress on Biotechnology
  • Int'l Joint Research
• [Presentation] 基板材料結合タンパク質を用いたナノデバイス指向インターフェイス分子開発 (2017)
  • Author(s): 宮木 達輝、中澤 光、筋野 拓馬、梅津 光央
  • Organizer: 第69回日本生物工学会
• [Presentation] Development of material-binding proteins fused device to improve sensitivity of biosensor (2017)
  • Author(s): Tatsuki Miyaki , HikaruNakazawa , Takuma Sujino , and Mitsuo Umetsu
  • Organizer: International Conference On Nanomedicine And Nanobiotechnology 2017
  • Int'l Joint Research
• [Presentation] A high-affinity material-binding camel antibody: antibody engineering for one-step functionalization of material surfaces (2017)
  • Author(s): Mitsuo Umetsu, Takamitsu Hattori, Takeshi Nakanishi, Satoko Sawai, Shinsuke Kikuchi, Ryutaro Asano, Izumi Kumagai
  • Organizer: 5th International Conference on Bio-Sensing Technology
  • Int'l Joint Research
• [Presentation] 材料表面特異的抗体から発想するプラズモニック膜と多孔質膜のボトムアップ作製プロセス (2017)
  • Author(s): 二井手 哲平, 真鍋 法義, 中澤 光・, 服部 峰充, 熊谷 泉, 梅津 光央
  • Organizer: 化学工学会第82回年会
  • Place of Presentation: 芝浦工業大学 (東京)
• [Presentation] Protein engineering for interface design on inorganic nanomaterials (2016)
  • Author(s): Mitsuo Umetsu
  • Organizer: 29th International Microprocesses and Nanotechnology Conference (MNC2016)
  • Place of Presentation: ANA Crowne Plaza Kyoto (Kyoto)
  • Int'l Joint Research / Invited