Development of intrabody selection technology in mammalian cells
| Project/Area Number |
16K14486
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Nagamune Teruyuki 東京大学, 大学院工学系研究科(工学部), 教授 (20124373)
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| Co-Investigator(Renkei-kenkyūsha) |
KAWAHARA Masahiro 東京大学, 大学院工学系研究科, 准教授 (50345097)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | バイオテクノロジー / キメラ受容体 / 細胞内抗体スクリーニング / 細胞内蛋白質間相互作用検出 / 細胞アレイ |
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| Outline of Final Research Achievements |
In this study, for the development of next-generation antibody drug, we developed a novel versatile intrabody selection method, which can select intrabody against antigen protein expressed in the cytosol of mammalian cells rapidly and directly. We constructed a chimeric receptor library, which can transduce growth signal by binding to antigen, by conjugating naive library of single chain antibody scFv and intracellular domain of receptor c-kit through flexible linker G4S. We expressed this chimeric receptor library together with a model antigen, the nucleoprotein of a rabies virus, in IL-3 dependent mammalian cell Ba/F3, and successfully obtained several scFv binders against the antigen protein from proliferated cells under IL-3 deficient culture condition.
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Report
(3 results)
Research Products
(19 results)
