| Project/Area Number |
16K14487
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
Tanaka Tsuyoshi 東京農工大学, 工学(系)研究科(研究院), 教授 (20345333)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | オイルボディ / 珪藻 / ディスプレイ / オイルボディー |
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| Outline of Final Research Achievements |
Novel design for the bioprocess of hydrophobic molecule production was achieved in this study. The oil accumulation organelle, oil body, in the oleaginous diatom Fistulifera solaris was utilized as a reaction and storage space for the hydrophobic molecule production. Toward this goal, first, oil body-associated proteins were identified by the proteome analysis of the oil body fraction extracted from F. solaris. Using an identified protein as an anchor molecule, oil body-display technology was established. Subsequently, production of hydrophobic polyunsatulated fatty acids (PUFAs) was enhanced by genetic engineering. It was suggested that the produced PUFAs were partitioned into the oil bodies in F. solaris. These techniques can contribute to the efficient production of various hydrophobic molecules in microalgae.
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