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Large-scale preparation of hair follicle germs and its application for hair regenerative medicine

Research Project

Project/Area Number 16K14489
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biofunction/Bioprocess
Research InstitutionYokohama National University

Principal Investigator

Fukuda Junji  横浜国立大学, 大学院工学研究院, 教授 (80431675)

Co-Investigator(Kenkyū-buntansha) 片岡 健  岡山理科大学, 理学部, 教授 (10293317)
鈴木 敦  横浜国立大学, 大学院工学研究院, 准教授 (60467058)
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords毛髪 / 再生医療 / 毛包 / 毛包原基 / マイクロデバイス / 脱毛症 / シリコーンゴム / 毛髪再生医療 / PDMS / 自己組織化 / 微細加工
Outline of Final Research Achievements

In this study, we developed a large-scale preparation approach based on self-organization of two types of cells and demonstrated that this approach is beneficial for hair regenerative medicine. We mixed two types of hair follicle derived cells and let them form an aggregate. They are initially randomly distributed in the aggregate but then spontaneously separated each other during 3 days of culture, resulting in the formation of hair follicle germs. Furthermore, we developed a microdevice in which a large number of hair follicle germs were formed through this self-organization. We demonstrated that this approach is applicable to human hair follicle derived cells as a proof of concept.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (20 results)

All 2018 2017 2016 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (11 results) (of which Int'l Joint Research: 4 results,  Invited: 1 results) Remarks (1 results) Patent(Industrial Property Rights) (5 results)

  • [Journal Article] Spontaneous hair follicle germ (HFG) formation in vitro, enabling the large-scale production of HFGs for regenerative medicine2018

    • Author(s)
      Kageyama T, Yoshimura C, Myasnikova D, Kataoka K, Nittami T, Maruo S, Fukuda J
    • Journal Title

      Biomaterials

      Volume: 154 Pages: 291-300

    • DOI

      10.1016/j.biomaterials.2017.10.056

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Flatbed epi relief-contrast cellular monitoring system for stable cell culture2017

    • Author(s)
      T. Osaki, T. Kageyama, Y. Shimazu, D. Mysnikova, S. Takahashi, S. Takimoto, J. Fukuda
    • Journal Title

      Scientific Reports

      Volume: n/a

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] In situ cross-linkable gelatin-CMC hydrogels designed for rapid engineering of perfusable vasculatures2016

    • Author(s)
      Tatsuto Kageyama, Tatsuya Osaki, Junko Enomoto, Dina Myasnikova, Tadashi Nittami, Takuro Hozumi, Taichi Ito, and Junji Fukuda
    • Journal Title

      ACS Biomaterials Science and Engineering

      Volume: 6 Issue: 6 Pages: 1059-1066

    • DOI

      10.1021/acsbiomaterials.6b00203

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] Novel approach for expansion of hair follicle stem cell by using PDMS microwell plate2018

    • Author(s)
      平野杉, 景山達斗,福田淳二
    • Organizer
      化学工学会第83年次会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Engineering skin tissue constructs with hair follicle germ2018

    • Author(s)
      楯芳樹, 景山達斗,福田淳二
    • Organizer
      化学工学会第83年次会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 脱毛症治療のためのin vitro毛髪再生2017

    • Author(s)
      清水亮啓、景山達斗、片岡健、平川聡史、福田淳二
    • Organizer
      第90回日本組織培養学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 毛包原基を用いた発毛剤スクリーニングツールの開発2017

    • Author(s)
      清水亮哲、景山達斗、平野杉、福田淳二
    • Organizer
      化学工学会第49回秋季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Engineering Tissue Fabrication Processes for Regenerative Medicine2017

    • Author(s)
      Junji Fukuda
    • Organizer
      TERMIS-AP 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Long-term culture of hair follicle germs formed through cell self-organization2017

    • Author(s)
      Akihiro Shimizu, Tatsuto Kageyama, Junji Fukuda
    • Organizer
      10th WCHR 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Large-scale production of hair follicle germ (HFG) using spontaneous HFG formation in vitro2017

    • Author(s)
      Chisa Yoshimura, Tatsuto Kageyama, Akihiro Shimizu, Lei Yan, Sugi Hirano, Yoshiki Tate, Keiichiro Kasai, Junji Fukuda
    • Organizer
      10th WCHR 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Large-scale preparation of hair follicle germs using oxygen-permeable PDMS microarray chips.2016

    • Author(s)
      Tatsuto Kageyama and Junji Fukuda
    • Organizer
      3rd International Conference on Biomaterial Science in Tokyo 2016 (ICBS2016)
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2016-11-28
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 微細加工技術を用いた毛包原基大量調製容器の開発2016

    • Author(s)
      景山達斗・吉村知紗・園山由希江・清水亮啓・Yan Lei・福田 淳二
    • Organizer
      第21回日本臨床毛髪学会学術集会
    • Place of Presentation
      はまぎんホール ヴィアマーレ
    • Year and Date
      2016-11-26
    • Related Report
      2016 Research-status Report
  • [Presentation] 間葉系細胞包埋ゲルビーズを用いた毛髪再生技術の開発2016

    • Author(s)
      景山達斗・福田 淳二
    • Organizer
      第54回日本人工臓器学会大会
    • Place of Presentation
      米子コンベンションセンター
    • Year and Date
      2016-11-23
    • Related Report
      2016 Research-status Report
  • [Presentation] 酸素透過性マイクロウェルアレイチップを用いた毛包原基の大量調製2016

    • Author(s)
      景山達斗、吉村知紗、片岡健、福田淳二
    • Organizer
      第67回日本生物工学会大会
    • Place of Presentation
      富山国際会議場
    • Year and Date
      2016-09-28
    • Related Report
      2016 Research-status Report
  • [Remarks] 研究室ホームページ

    • URL

      http://www.fukulab.ynu.ac.jp/

    • Related Report
      2017 Annual Research Report
  • [Patent(Industrial Property Rights)] 色素の蓄積を制御する候補化合物をスクリーニングする方法及びキット2018

    • Inventor(s)
      福田淳二、景山達斗、清水亮啓
    • Industrial Property Rights Holder
      福田淳二、景山達斗、清水亮啓
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2018-004432
    • Filing Date
      2018
    • Related Report
      2017 Annual Research Report
  • [Patent(Industrial Property Rights)] 毛髪再生用キット及び発毛促進又は抑制物質をスクリーニングする方法2017

    • Inventor(s)
      福田淳二、景山達斗、清水亮啓
    • Industrial Property Rights Holder
      福田淳二、景山達斗、清水亮啓
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2017-159661
    • Filing Date
      2017
    • Related Report
      2017 Annual Research Report
  • [Patent(Industrial Property Rights)] 再生毛包原基を有する培養皮膚の製造方法及びその使用2017

    • Inventor(s)
      福田淳二、景山達斗、楯芳樹
    • Industrial Property Rights Holder
      福田淳二、景山達斗、楯芳樹
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2017-217654
    • Filing Date
      2017
    • Related Report
      2017 Annual Research Report
  • [Patent(Industrial Property Rights)] 毛細血管構造を有する再生毛包原基の集合体の製造方法2016

    • Inventor(s)
      福田淳二、景山達斗
    • Industrial Property Rights Holder
      横浜国立大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2016-226344
    • Filing Date
      2016-11-21
    • Related Report
      2016 Research-status Report
  • [Patent(Industrial Property Rights)] 毛髪再生用細胞包埋ビーズ及びその製造方法2016

    • Inventor(s)
      福田淳二、景山達斗
    • Industrial Property Rights Holder
      横浜国立大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2016-120808
    • Filing Date
      2016-06-17
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2019-03-29  

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