Development of methods for the enzymatic synthesis of 18O-phosphate
| Project/Area Number |
16K14492
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Hiroshima University |
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Principal Investigator |
Kuroda Akio 広島大学, 先端物質科学研究科, 教授 (50205241)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 酸素の安定同位体リン酸 / 酵素合成法 / 亜リン酸デヒドロゲナーゼ / ピロホスファターゼ / ポリホスファターゼ / 酸素-18安定同位体リン酸 / 亜リン酸 / ポリリン酸 / 動態モニタリング / ラベリング / トレーサー |
|---|
| Outline of Final Research Achievements |
Due to the lack of multiple stable isotopes of phosphorus (P), it is difficult to use isotope pair ratios as a means to examine P dynamics in nature. However, if stable isotope of oxygen (18O) is incorporated into phosphate, 18O-phosphate can be used to trace P dynamics. In order to synthesize 18O-phosphate where the number of 18O substitution over 16O is different, we tested several enzymatic reactions. For example, phosphite dehydrogenase is an enzyme to oxidize phosphite to phosphate with a NAD cofactor. The 18O of 18O-H2O was incorporated into phosphite, generating 18O-phosphate. On the contrary, all four oxygen of phosphate was substituted with 18O of 18O-H2O using pyrophosphatase that converts pyrophosphate to phosphate in a reversible manner.
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Report
(3 results)
Research Products
(6 results)
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[Book] リンの事典2017
Author(s)
黒田章夫、大竹久夫他
Total Pages
360
Publisher
朝倉書店
ISBN
9784254141047
Related Report
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