| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Amide compounds are useful materials as bio-functional molecules and chemicals. We have developed a unique method for amino acid amide synthesis using adenylation domain (A-domain) of nonribosomal peptide synthetase. In this method, A-domain adenylates carboxy group of amino acid and followed by nucleophilic acyl substitution reaction with amine. In this research, novel methods of synthesizing various amide compounds using adenylation enzymes were developed.
Fatty acid amides were synthesized by a fatty acyl-AMP ligase which can adenylate various length of fatty acids. Aryl carboxylic acid amides were synthesized by DhbE which can adenylate benzoic acid and 14 kinds of monosubstituted benzoic acids. D-Amino acid-containing dipeptides were synthesized by A-domain (TycA-A) which can adenylate some D-amino acids. On the other hand, the ATP regeneration system from AMP was developed using class III polyphosphate kinase 2 and was coupled with L-Trp-L-Pro synthesis using TycA-A.
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