Construction method for comlicated gene circuits by multi-step cell fusion

• Project/Area Number: 16K14497
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biofunction/Bioprocess
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Fukuda Nobuo 国立研究開発法人産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (00613548)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: 酵母 / 接合 / 遺伝子パーツ / 細胞融合 / 遺伝子回路

Outline of Final Research Achievements

Synthetic biology aims to create advanced vital functions by combining circuits composed of genetic parts as well as integration methods of electronic circuits to form complexes. In this study, we succeeded in establishing a technology for stepwise integration of genetic parts by cell fusion (mating) of yeast cells having certain functional units. Since this technology can be widely applied to general laboratory yeasts used for academic research, it is possible to make full use of abundant resources existing in gene banks and the like.

Research Products

• [Journal Article] Positive Detection of GPCR Antagonists Using a System for Inverted Expression of a Fluorescent Reporter Gene. (2017)
  • Author(s): Fukuda N, Kaishima M, Ishii J, Honda S
  • Journal Title: ACS synthetic biology Volume: 6 Issue: 8 Pages: 1554-1562
  • DOI: 10.1021/acssynbio.7b00056
  • Peer Reviewed
• [Journal Article] Continuous crossbreeding of sake yeasts using growth selection systems for a-type and α-type cells (2016)
  • Author(s): Fukuda N, Kaishima M, Ishii J, Kondo A, Honda S
  • Journal Title: AMB Express Volume: 6 Issue: 1 Pages: 1-12
  • DOI: 10.1186/s13568-016-0216-x
  • Peer Reviewed / Open Access
• [Journal Article] Expression of varied GFPs in Saccharomyces cerevisiae: codon optimization yields stronger than expected expression and fluorescence intensity. (2016)
  • Author(s): Kaishima M, Ishii J, Matsuno T, Fukuda N, Kondo A
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 35932-35932
  • DOI: 10.1038/srep35932
  • Peer Reviewed / Open Access
• [Presentation] GPCR拮抗薬のポジティブ検出を可能とする反転型レポーター発現系 (2017)
  • Author(s): 福田 展雄、石井 純、本田 真也
  • Organizer: 第69回日本生物工学会大会
• [Presentation] 胞子形成過程を回避した酵母の人為的有性生殖法 (2016)
  • Author(s): 福田 展雄
  • Organizer: 第68回日本生物工学会大会
  • Place of Presentation: 富山国際会議場(富山市)
• [Presentation] Selection systems for a-type and α-type yeasts using cell-type-specific transcriptional regulation machinery (2016)
  • Author(s): 福田 展雄
  • Organizer: 2nd International Conference on Systems and Synthetic Biology
  • Place of Presentation: Crowne Plaza Heathrow（London, UK）
  • Int'l Joint Research