| Project/Area Number |
16K14498
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
Fujita Satoshi 国立研究開発法人産業技術総合研究所, バイオメディカル研究部門, 研究グループ長 (00392655)
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| Co-Investigator(Renkei-kenkyūsha) |
Sugiura Shinji 国立研究開発法人 産業技術総合研究所, 生命工学領域 創薬基盤研究部門 医薬品アッセイデバイス研究グループ, 上級主任研究員 (00392655)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 遺伝子導入 / 表面化学 / マイクロ・ナノバイオプロセス / マイクロ・ナノデバイス / マイクロアレイ |
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| Outline of Final Research Achievements |
We attempted to make the interface of multi-layered genes where plasmids encoding different genes are transfected into cells by controlling timing. Plasmid expressing a fluorescent protein was encapsulated into cationic lipid to make cationic lipoplex. And multi-layered interface on glass slide was made by the cationic lipoplex, PLL and anionic hyaluronic acid. After seeding cells on the interface, we checked transfection timing of genes. As a result, we found transfection timing between the gene in upper layer and in lower layer is different for more than 20 hours.
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