Development of novel synaptic red chloride probe
Project/Area Number |
16K14552
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Neurophysiology / General neuroscience
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Research Institution | Saitama University |
Principal Investigator |
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Project Period (FY) |
2016-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 神経科学 / 神経活動 / 抑制性シナプス / クロライドプローブ / 蛍光イメージング |
Outline of Final Research Achievements |
Increases in intracellular chloride ion concentrations of neurons following inhibitory postsynaptic potentials (IPSPs) have not been visualized in vivo to date. In this study, aiming at detecting IPSPs in vivo, we developed a red fluorescent genetically encoded chloride probe with high responsivity. Then we expressed the probe in neuronal cells and assessed its functionalities, focusing on responses to inhibitory inputs.
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Report
(3 results)
Research Products
(52 results)
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[Journal Article] An R-CaMP1.07 reporter mouse for cell-type-specific expression of a sensitive red fluorescent calcium indicator.2017
Author(s)
Bethge P, Carta S, Lorenzo DA, Egolf L, Goniotaki D, Madisen L, Voigt FF, Chen JL, Schneider B, Ohkura M, Nakai J, Zeng H, Aguzzi A, Helmchen F
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Journal Title
PLoS One
Volume: 12:e0179460
Issue: 6
Pages: 1-19
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Allogeneic transplantation of iPS cell-derived cardiomyocytes regenerates primate hearts.2016
Author(s)
Shiba Y, Gomibuchi T, Seto T, Wada Y, Ichimura H, Tanaka Y, Ogasawara T, Okada K, Shiba N, Sakamoto K, Ido D, Shiina T, Ohkura M, Nakai J, Uno N, Kazuki Y, Oshimura M, Minami I, Ikeda U
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Journal Title
Nature
Volume: 538
Issue: 7625
Pages: 388-391
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Olfactory receptor for prostaglandin F2α mediates male fish courtship behavior.2016
Author(s)
Yabuki Y, Koide T, Miyasaka N, Wakisaka N, Masuda M, Ohkura M, Nakai J, Tsuge K, Tsuchiya S, Sugimoto Y, Yoshihara Y
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Journal Title
Nature Neuroscience
Volume: 19
Issue: 7
Pages: 897-904
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Unraveling the dynamism of engram cells during contextual memory processing.2017
Author(s)
Ghandour K, Ohkawa N, Fung CCA, Saitoh Y, Takekawa T, Asai H, Okubo-Suzuki R, Nomoto M, Soya S, Tsujimura S, Nishizono H, Matsuo M, Sato M, Ohkura M, Nakai J, Hayashi Y, Sakurai T, Osanai M, Fukai T, Inokuchi K
Organizer
Society for Neuroscience 2017 Annual Meeting
Related Report
Int'l Joint Research
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[Presentation] Functional breakdown processes of neural circuits in hippocampal CA1 region of Alzheimer’s disease model mice.2017
Author(s)
Takamura R, Mizuta K, Sekine Y, Islam T, Saito T, Takekawa T, Ohkura M, Fukai T, Nakai J, Saido TC, Hayashi Y
Organizer
Society for Neuroscience 2017 Annual Meeting
Related Report
Int'l Joint Research
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[Presentation] Cellular mechanisms for the formation and plasticity of hippocampal cognitive maps.2017
Author(s)
Sato M, Mizuta K, Islam T, Kawano M, Takekawa T, Gomez-Dominguez D, Yamakawa H, Ohkura M, Fukai T, Nakai J, Hayashi Y
Organizer
Society for Neuroscience 2017 Annual Meeting
Related Report
Int'l Joint Research
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[Presentation] Ultra-thin fluorescence endoscope imaging system for functional biological imaging.2017
Author(s)
Osanai M, Ohkawa N, Kamiya A, Inami C, Sato M, Ohkura M, Kojima T, Kohmura Y, Hayashi Y, Yanagawa Y, Ohsawa M, Nakai J, Inokuchi K, Homma N, Mushiake H
Organizer
計測自動制御学会 システム・情報部門 学術講演会 2017
Related Report
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[Presentation] 頭部固定コモンマーモセット一次運動野における前肢運動課題実行中の2光子イメージング.2017
Author(s)
蝦名鉄平, 正水芳人, 平川玲子, 渡我部昭哉, 大倉正道, 小林憲太, 纐纈大輔, 南部篤, 彦坂和雄, 佐々木えりか, 中井淳一, 山森哲雄, 松崎政紀
Organizer
第40回日本神経科学大会
Related Report
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[Presentation] 海馬CA1セルアセンブリによる報酬事象表現.2017
Author(s)
水田恒太郎, 佐藤正晃, 関根友紀子, 河野真子, イスラム タンビル, 高村理沙, 竹川嵩志, 大倉正道, 深井朋樹, 中井淳一, 林康紀
Organizer
第40回日本神経科学大会
Related Report
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[Presentation] 脳機能イメージングのための極微細蛍光内視鏡イメージングシステム.2017
Author(s)
小山内実, 大川宜昭, 坂本一寛, 三輪秀樹, 菊田里美, 田村篤史, 佐藤正晃, 大倉正道, 小島太郎, 幸村裕治, 中井淳一, 林康紀, 柳川右千夫, 井ノ口馨, 本間経康, 虫明元
Organizer
第40回日本神経科学大会
Related Report
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[Presentation] Identification of characteristic dynamism of engram cells during learning.2017
Author(s)
Ghandour K, Ohkawa N, Fung CCA, Saitoh Y, Takekawa T, Asai H, Okubo-Suzuki R, Nomoto M, Tsujimura S, Nishizono H, Matsuo M, Sato M, Ohkura M, Nakai J, Hayashi Y, Fukai T, Inokuchi K
Organizer
第40回日本神経科学大会
Related Report
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[Presentation] 海馬認知地図形成の神経細胞ダイナミクス.2017
Author(s)
佐藤正晃, 水田恒太郎, Islam T, 河野真子, 竹川高志, Gomez-Dominguez D, 山川宏, 大倉正道, 深井朋樹, 中井淳一, 林康紀
Organizer
第40回日本神経科学大会
Related Report
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[Presentation] アルツハイマー病モデルマウス海馬CA1領域における神経機能回路の破綻過程.2017
Author(s)
高村理沙, 水田恒太郎, 関根友紀子, Islam T, 斉藤貴志, 竹川高志, 大倉正道, 深井朋樹, 中井淳一, 西道隆臣, 林康紀
Organizer
第40回日本神経科学大会
Related Report
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[Presentation] Temporal coding of reward event by subpopulations of hippocampal CA1 pyramidal neurons.2016
Author(s)
K. Mizuta, M. Sato, Y. Sekine, M. Kawano, T. Islam, R. Takamura, T. Masumoto, T. Takekawa, M. Ohkura, T. Fukai, J. Nakai, Y. Hayashi
Organizer
Society for Neuroscience 2016 Annual Meeting
Place of Presentation
San Diego Convention Center(San Diego, USA)
Year and Date
2016-11-12
Related Report
Int'l Joint Research
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[Presentation] Preferential stabilization of behaviorally relevant spatial representations in the hippocampal place map.2016
Author(s)
M. Sato, K. Mizuta, T. Islam, M. Kawano, T. Takekawa, D. Gomez-Dominguez, H. Yamakawa, M. Ohkura, T. Fukai, J. Nakai, Y. Hayashi
Organizer
Society for Neuroscience 2016 Annual Meeting
Place of Presentation
San Diego Convention Center(San Diego, USA)
Year and Date
2016-11-12
Related Report
Int'l Joint Research
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[Presentation] A TIGRE reporter mouse line driving Cre- and tTA-dependent expression of the red calcium indicator R-CaMP1.07.2016
Author(s)
P. Bethge, L. Egolf, D.A. Lorenzo, D. Goniotaki, L. Madisen, F.F. Voigt, M. Ohkura, J. Nakai, H. Zeng, A. Aguzzi, F. Helmchen
Organizer
Society for Neuroscience 2016 Annual Meeting
Place of Presentation
San Diego Convention Center(San Diego, USA)
Year and Date
2016-11-12
Related Report
Int'l Joint Research
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[Presentation] 行動上重要な場所の表象は海馬CA1場所地図において優先的に安定化される.2016
Author(s)
佐藤正晃, 水田恒太郎, Tanvir Islam, 河野真子, 竹川高志, Daniel Gomez-Dominguez, 山川宏, 大倉正道, 深井朋樹, 中井淳一, 林康紀
Organizer
第39回日本神経科学大会
Place of Presentation
パシフィコ横浜(神奈川県横浜市)
Year and Date
2016-07-20
Related Report
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[Presentation] 経頭蓋直流電気刺激が誘起する可塑性におけるグリア細胞の関与.2016
Author(s)
毛内拡, 大倉正道, 田中三佳, 大江佑樹, 今野歩, 平井宏和, 御子柴克彦, 糸原重美, 中井淳一, 岩井陽一, 平瀬肇
Organizer
第39回日本神経科学大会
Place of Presentation
パシフィコ横浜(神奈川県横浜市)
Year and Date
2016-07-20
Related Report
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