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Development of a versatile vector system for single-cell labeling and gene function studies in vivo

Research Project

Project/Area Number 16K14559
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Neurophysiology / General neuroscience
Research InstitutionNational Institute of Genetics

Principal Investigator

IWASATO Takuji  国立遺伝学研究所, 遺伝形質研究系, 教授 (00311332)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords神経科学 / 神経回路 / 単一細胞解析 / 細胞標識 / 遺伝子ノックアウト / 発達 / マウス / 遺伝学 / 脳 / 新規手法 / 遺伝子 / ノックアウト / 蛍光標識 / in vivo / 哺乳類
Outline of Final Research Achievements

We developed “Supernova” that is a versatile vector system for single-cell labeling and gene function studies in vivo. Supernova system enables single-cell labeling and labeled cell-specific gene manipulation, when introduced by in utero electroporation (IUE) or adeno-associated virus (AAV).

Academic Significance and Societal Importance of the Research Achievements

遺伝子を操作するためにTALEN, CRISPR/Cas9などの最新のゲノム編集技術を取り入れることで、高性能で汎用性のあるSupernovaシリーズを構築した。その結果、遺伝子組換え動物ではない多くのモデル生物においても単一細胞遺伝子ノックアウトできるようになった。また、ベクター導入に、これまでの子宮内電気穿孔法に加えてアデノ随伴ウイルス(AAV)が利用できるようになり、幅広い組織への適用が可能になった。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (28 results)

All 2019 2018 2017 2016

All Journal Article (10 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 10 results,  Open Access: 8 results,  Acknowledgement Compliant: 1 results) Presentation (17 results) (of which Int'l Joint Research: 6 results,  Invited: 16 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] In Vivo Two-photon Imaging of Cortical Neurons in Neonatal Mice2018

    • Author(s)
      Mizuno H*, Nakazawa S, Iwasato T (* correspondence)
    • Journal Title

      Journal of Visualized Experiments

      Volume: 140 Issue: 140

    • DOI

      10.3791/58340

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Differential dynamics of cortical neuron dendritic trees revealed by long-term in vivo imaging in neonates2018

    • Author(s)
      Nakazawa Shingo、Mizuno Hidenobu、Iwasato Takuji
    • Journal Title

      Nature Communications

      Volume: 9 Issue: 1 Pages: 3106-3106

    • DOI

      10.1038/s41467-018-05563-0

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of tactile sensory circuits in the CNS2018

    • Author(s)
      Iwasato Takuji、Erzurumlu Reha S.
    • Journal Title

      Current Opinion in Neurobiology

      Volume: 53 Pages: 66-75

    • DOI

      10.1016/j.conb.2018.06.001

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Patchwork-type spontaneous activity in neonatal barrel cortex layer 4 transmitted via thalamocortical projections.2018

    • Author(s)
      Mizuno, H., Ikezoe, K., Nakazawa, S., Sato, T., Kitamura, K., Iwasato, T.
    • Journal Title

      Cell Rep.

      Volume: 22 Issue: 1 Pages: 123-135

    • DOI

      10.1016/j.celrep.2017.12.012

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Protocadherin-αC2 is required for diffuse projections of serotonergic axons2017

    • Author(s)
      Katori Shota、Noguchi-Katori Yukiko、Okayama Atsushi、Kawamura Yoshimi、Luo Wenshu、Sakimura Kenji、Hirabayashi Takahiro、Iwasato Takuji、Yagi Takeshi
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 15908-15908

    • DOI

      10.1038/s41598-017-16120-y

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Spinal RacGAP α-Chimaerin Is Required to Establish the Midline Barrier for Proper Corticospinal Axon Guidance2017

    • Author(s)
      Katori Shota、Noguchi-Katori Yukiko、Itohara Shigeyoshi、Iwasato Takuji
    • Journal Title

      The Journal of Neuroscience

      Volume: 37 Issue: 32 Pages: 7682-7699

    • DOI

      10.1523/jneurosci.3123-16.2017

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Prenatal thalamic waves regulate cortical area size prior to sensory processing.2017

    • Author(s)
      Moreno-Juan, V., Filipchuk, A., Anton-Bolarios, N., Mezzera, C., Gezelius, H., Andes, B., Rodriguez-Malmierca, L., Susin, R., Schaad, O., Iwasato, T., Schuele, R., Rutlin, M., Nelson, S., Ducret, S., Valdeolmillos, M., Rijli, F., Lopez-Bendito, G.
    • Journal Title

      Nature Commun.

      Volume: 8 Issue: 1 Pages: 14172-14172

    • DOI

      10.1038/ncomms14172

    • Related Report
      2017 Research-status Report 2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo.2016

    • Author(s)
      Luo, W., Mizuno, H., Iwata, R., Nakazawa, S., Yasuda, K., Itohara, S., Iwasato, T.
    • Journal Title

      Sci Rep.

      Volume: 6 Issue: 1 Pages: 35747-35747

    • DOI

      10.1038/srep35747

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] CAPS1 stabilizes the state of readily releasable synaptic vesicles to fusion competence at CA3-CA1 synapses in adult hippocampus2016

    • Author(s)
      6.Shinoda Y, Ishii C, Fukazawa Y, Sadakata T, Ishii Y, Sano Y, Iwasato T, Itohara S, Furuichi T
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 31540-31540

    • DOI

      10.1038/srep31540

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] In vitro Assay for Dendritic Spine Retraction of Hippocampal Neurons with Sparse Labeling2016

    • Author(s)
      Iwata, R., Iwasato, T.
    • Journal Title

      Bio-Protocol

      Volume: 6

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 赤ん坊の脳の中での神経回路の発達 ―マウス遺伝学と二光子イメージングを用いた研究2019

    • Author(s)
      岩里琢治
    • Organizer
      第18回BIRD脳科学セミナー
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] In vivo imaging of barrel cortex layer 4 during thalamocortical reorganization2018

    • Author(s)
      Takuji Iwasato
    • Organizer
      遺伝研研究会2018 “Circuit construction in the mammalian brain”
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] In vivo imaging of barrel cortex circuit refinement in neonates2018

    • Author(s)
      Takuji Iwasato
    • Organizer
      Spontaneous Activity in Brain Development (SPONT 2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] In vivo functional and morphological imaging of neural circuit refinement in the neonatal barrel cortex2018

    • Author(s)
      岩里琢治
    • Organizer
      CSHA(Cold Spring Harbor Asia) on Neuroscience 2018 「Latest Advances in Development and Function of Neuronal Circuits」サテライトシンポジウム
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 新生仔マウスのバレル皮質神経回路発達のin vivoイメージング (In vivo imaging of barrel cortex circuit refinement in neonates)2018

    • Author(s)
      岩里琢治
    • Organizer
      第41回日本神経科学大会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] マウスで迫る「赤ん坊の脳の発達のしくみ」2018

    • Author(s)
      岩里琢治
    • Organizer
      JT生命誌研究館・国立遺伝学研究所共同企画シンポジウム「遺伝学の最先端研究から見る生命誌」
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] マウス遺伝学を用いた体性感覚系神経回路発達の解析2017

    • Author(s)
      岩里琢治
    • Organizer
      新潟大学脳研究所共同研究合同セミナー
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Unveiling the mechanisms of neuronal circuit refinement in the neonatal mouse barrel Cortex2017

    • Author(s)
      岩里琢治
    • Organizer
      Academia Sinica Symposium
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] マウス大脳皮質の生後発達期神経回路リモデリング2017

    • Author(s)
      岩里琢治
    • Organizer
      第12回「認識と形成」研究会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 生後発達期の大脳皮質リモデリング2017

    • Author(s)
      岩里琢治
    • Organizer
      生理学研究所研究会「大脳皮質回路の機能原理を探る」
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] マウス大脳皮質の生後発達期神経回路リモデリング2017

    • Author(s)
      岩里琢治
    • Organizer
      第5回「幸福脳」研究会(名古屋大学・慶応大学合同ミーティング)
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 哺乳類中枢神経系における神経回路形成の遺伝学的解析2017

    • Author(s)
      岩里琢治
    • Organizer
      新潟大学脳研究所共同研究合同セミナー
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] In vivo imaging of neonatal mouse barrel cortex2016

    • Author(s)
      T. Iwasato
    • Organizer
      Dart Neuroscience Seminar Series at TSRI
    • Place of Presentation
      SD, USA
    • Year and Date
      2016-11-18
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Supernova: Extensible vector systems that enable high intensity single-cell labeling and labeled cell-specific gene knockout and editing In vivo2016

    • Author(s)
      S. Nakazawa., W. Luo., R. Iwata., H. Mizuno., T. Iwasato.
    • Organizer
      Society for Neuroscience 2016
    • Place of Presentation
      SD, USA
    • Year and Date
      2016-11-12
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 独自の二光子イメージング技術を用いた、新生仔マウス大脳皮質における神経回路発達の解析2016

    • Author(s)
      岩里琢治
    • Organizer
      第159回日本獣医学会学術集会
    • Place of Presentation
      藤沢
    • Year and Date
      2016-09-07
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] In vivo imaging of neonatal mouse barrel cortex2016

    • Author(s)
      T. Iwasato.
    • Organizer
      Circuit Construction in the Mammalian Brain
    • Place of Presentation
      大阪
    • Year and Date
      2016-08-08
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 単一細胞蛍光標識と標識細胞特異的遺伝子操作を可能とするベクターシステム2016

    • Author(s)
      岩里琢治
    • Organizer
      第13回アカデミックフォーラム
    • Place of Presentation
      東京
    • Year and Date
      2016-05-13
    • Related Report
      2016 Research-status Report
    • Invited
  • [Patent(Industrial Property Rights)] ベクターシステム、遺伝子発現方法、標的遺伝子ノックアウト方法、標的遺伝子ノックダウン方法、標的遺伝子編集方法、及び遺伝子発現キット2016

    • Inventor(s)
      岩里琢治、水野秀信、羅ブンジュウ、岩田亮平
    • Industrial Property Rights Holder
      情報・システム研究機構
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2016-094814
    • Filing Date
      2016-05-10
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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