| Project/Area Number |
16K14566
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | University of Fukui |
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Principal Investigator |
Saito Harumi 福井大学, 学術研究院医学系部門, 学術研究員 (20311342)
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| Project Period (FY) |
2016-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Keywords | オプトジェネテイックス / 単一の嗅覚受容体 / 嗅皮質 / 情動行動 / 神経科学 |
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| Outline of Final Research Achievements |
Sensing of odorants is one of the most important sensory functions for animals to make behavioral decisions. We have made a mouse model system that can specifically elicit immobility responses behavior by the activation of single glomerular spicies in main olfactory system.
I have isolated a single olfactory receptor (ORTMT) that is specifically activated by the predator odor 2,4,5-dehydro-2,5 trimethylthiazoline (TMT) and generated ORTMT gene knock-out (KO) mice and knock-in channelrhodopsin (KI) mice which expressing in a pair of glomeruli that express an ORTMT and performed the loss / gain of function. The data indicated that the activation of ORTMT induced specifically a freezing but not aversive behavior. I assumed that this model mouse system targeting a single type of OR is useful to study the neural network and plan to proceed the study in the higher brain region.
Furthermore I set up the experiment system for loss of function of olfactory cortex.
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