| Project/Area Number |
16K14568
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Kyushu University (2017)
Institute of Physical and Chemical Research (2016) |
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Principal Investigator |
Imai Takeshi 九州大学, 医学研究院, 教授 (70509851)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Keywords | 超解像顕微鏡 / コネクトミクス / シナプス / 樹状突起スパイン / 生後発達 / スパイン / コネクトーム / 思春期 / 組織透明化 / 神経回路 |
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| Outline of Final Research Achievements |
It is generally believed that the number of dendritic spines increases during childhood, and then declines during adolescence to form mature neuronal circuits. However, distribution of dendritic spines at a whole-neuron scale is not fully established. Here we performed comprehensive super-resolution mapping of dendritic spines in layer 5 cortical pyramidal neurons in mice. We found that spine density is highly biased at the middle compartment of long apical dendrites (spine density “hotspot”), demonstrating ~10-fold accumulations. In contrast, the spine density was less biased in layer 2/3 neurons and basal dendrites of layer 5 neurons. We also found that spine density at the hotspot increased during adolescence in an NMDAR-dependent manner, when other parts of the dendrites underwent moderate reduction. Thus, the spine density is “reorganized”, rather than simply decreased during adolescence to form mature cortical circuits.
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