• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The experimental attempt for brain evolution by increase of neurons in mouse cerebral cortex

Research Project

Project/Area Number 16K14569
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Nerve anatomy/Neuropathology
Research InstitutionTokyo Metropolitan Institute of Medical Science

Principal Investigator

OKADO haruo  公益財団法人東京都医学総合研究所, 脳発達・神経再生研究分野, プロジェクトリーダー (60221842)

Co-Investigator(Kenkyū-buntansha) 平井 志伸  公益財団法人東京都医学総合研究所, 脳発達・神経再生研究分野, 研究員 (00625189)
Co-Investigator(Renkei-kenkyūsha) TANAKA Kenji  慶應義塾大学, 生物学的精神医学, 特任准教授 (30329700)
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsRP58 / 大脳皮質 / RP58 大脳新皮質 外側脳室下帯OSVZ / 大脳新皮質 / 外側脳室下帯OSVZ / OSVZ / AAV / 外側脳室下帯 / 脳進化 / 細胞周期離脱
Outline of Final Research Achievements

RP58 KO mice display an increase in progenitor cells due to inhibition of cell cycle exit. RP58 KO cortex shows a three-layered structure with an outer Pax6 layer, which resembles the primate outer subventricular zone (OSVZ). The OSVZ is known to important for increase of cortical neurons in the primate brain. We propose that a primate-like cortex can be generated from a rodent cortex by modulating cell cycle exit of progenitor cells. For example, inhibition of RP58 activity early in development may increase progenitor cell number leading to the formation of an OSVZ. Subsequent activation of RP58 may increase differentiation of mature neurons leading to formation of a large cortex with gyruses.
To examine the hypothesis, we tried to rescue these cells by RP58 supply at good timing using in utero electropolation or adenovirus vectors. However, we found these methods are insufficient in efficiency. Now we start FAST system for regulation of RP58 transcription.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (10 results)

All 2017 2016 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 3 results) Presentation (4 results) Remarks (2 results)

  • [Journal Article] AMPA glutamate receptors are required for sensory-organ formation and morphogenesis in the basal chordate.2017

    • Author(s)
      Hirai S, Hotta K, Kubo Y, Nishino A, Okabe S, Okamura Y, Okado H.
    • Journal Title

      Proc Natl Acad Sci U S A

      Volume: 114 Pages: 3939-3944

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] AMPA glutamate receptors are required for sensory-organ formation and morphogenesis in the basal chordate.2017

    • Author(s)
      Shinobu Hirai, Kohji Hotta, Yoshihiro Kubo, Atsuo Nishino, Shigeo Okabe, Yasushi Okamura, Haruo Okado
    • Journal Title

      Proc. Natl. Acad. Sci. U. S. A.

      Volume: - Issue: 15 Pages: 3939-3944

    • DOI

      10.1073/pnas.1612943114

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Altered tau isoform ratio caused by loss of Fus and Sfpq function leads to FTLD-like phenotypes2017

    • Author(s)
      Ishigaki S, Fujioka Y, Okada Y, Riku Y, Udagawa T, Honda D, Yokoi S, Endo K, Ikenaka K, Takagi S, Iguchi Y, Sahara N, Takashima A, Okano H, Yoshida M, Warita H, Aoki M, Watanabe H, Okado H, Katsuno H, Sobue G.
    • Journal Title

      Cell Reports

      Volume: 18 Issue: 5 Pages: 1118-1131

    • DOI

      10.1016/j.celrep.2017.01.013

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Phosphorylation of TAR DNA-binding Protein of 43 kDa (TDP-43) by Truncated Casein Kinase 1δ Triggers Mislocalization and Accumulation of TDP-43.2016

    • Author(s)
      Nonaka T, Suzuki G, Tanaka Y, Kametani F, Hirai S, Okado H, Miyashita T, Saitoe M, Akiyama H, Masai H, Hasegawa M.
    • Journal Title

      The journal of Biological Chemistry

      Volume: 291 Issue: 11 Pages: 5473-83

    • DOI

      10.1074/jbc.m115.695379

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 転写抑制因子RP58に結合する蛋白の網羅的な探索2016

    • Author(s)
      高沢 克子, 平井 清華, 進藤 真由美, 新保 裕子, 岡戸 晴生
    • Organizer
      第39回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-12-01
    • Related Report
      2016 Research-status Report
  • [Presentation] RP58の適切なバリアントと発現量が脳発達に重要である2016

    • Author(s)
      新保裕子、平井志伸、神嵜誠司 松本良江、田中謙二、岡戸晴生
    • Organizer
      第39回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report
  • [Presentation] 知的障害を持つ患者群から発見されたRP58/ZNF238変異体の機能解析2016

    • Author(s)
      神嵜誠司、平井志伸、新保裕子、岡戸晴生
    • Organizer
      第39回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-07-22
    • Related Report
      2016 Research-status Report
  • [Presentation] 選択的欠失によるRP58/ZNF238 のバイアント選る脳形態および脳機能異常2016

    • Author(s)
      新保裕子、平井志伸、神嵜誠司、田中謙二、岡戸晴生
    • Organizer
      第39回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-07-22
    • Related Report
      2016 Research-status Report
  • [Remarks] 東京都医学総合研究所神経細胞分化プロジェクト

    • URL

      http://www.igakuken.or.jp/project/detail/differentiation.html

    • Related Report
      2017 Annual Research Report
  • [Remarks] 東京都医学総合研究所

    • URL

      http://www.igakuken.or.jp

    • Related Report
      2017 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi