Study to understand mechanism of hemorrhagic stroke induced by oral bacteria

• Project/Area Number: 16K14573
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: National Cardiovascular Center Research Institute
• Principal Investigator: IHARA MASAFUMI 国立研究開発法人国立循環器病研究センター, 病院, 部長 (00372590)
• Co-Investigator(Kenkyū-buntansha): 仲野 和彦 大阪大学, 歯学研究科, 教授 (00379083) ; 齊藤 聡 国立研究開発法人国立循環器病研究センター, 病院, 医師 (40732996)
• Research Collaborator: SAITO Satoshi ; TONOMURA Shuichi
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 口腔内細菌 / ミュータンス菌 / 脳出血 / 脳微小出血 / 脳内微小出血 / 連鎖球菌 / 脳循環障害 / 齲蝕原性細菌 / コラーゲン結合性

Outline of Final Research Achievements

We investigated possible associations between collagen-bindin protein Cnm-positive Streptococcus mutans and cerebral hemorrhage. We enrolled 165 stroke patients who were assessed with brain MRI in the acute phase and found that those who had Cnm-positive Streptococcus mutans in the oral cavity had significantly greater nmumber of deep microbleeds in the brain. We are planning a prospective clinical research which will longitudinally evaluate possible increase of cerebral microbleeds to promote a new strategy of stroke prevention.

Research Products

• [Int'l Joint Research] ナイロビ大学(ケニア)
• [Int'l Joint Research] イバダン大学(ナイジェリア)
• [Journal Article] Chronic cerebral hypoperfusion: a key mechanism leading to vascular cognitive impairment and dementia. Closing the translational gap between rodent models and human vascular cognitive impairment and dementia (2017)
  • Author(s): Duncombe Jessica、Kitamura Akihiro、Hase Yoshiki、Ihara Masafumi、Kalaria Raj?N.、Horsburgh Karen
  • Journal Title: Clinical Science Volume: 131 Issue: 19 Pages: 2451-2468
  • DOI: 10.1042/cs20160727
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Oral Cnm-positive Streptococcus Mutans Expressing Collagen Binding Activity is a Risk Factor for Cerebral Microbleeds and Cognitive Impairment. (2016)
  • Author(s): Watanabe I, Kuriyama N, Miyatani F, Nomura R, Naka S, Nakano K, Ihara M, Iwai K, Matsui D, Ozaki E, Koyama T, Nishigaki M, Yamamoto T, Tamura A, Mizuno T, Akazawa K6, Takada A, Takeda K, Yamada K, Nakagawa M, Tanaka T, Kanamura N, Friedland RP, Watanabe Y.
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 1-5
  • DOI: 10.1038/srep38561
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] う蝕原因菌と脳出血の関連－脳口連関－． (2016)
  • Author(s): 殿村修一，仲野和彦，猪原匡史
  • Journal Title: 日本歯科論評 Volume: 76 Pages: 14-15
• [Presentation] Vascular Dementia (2017)
  • Author(s): Masafumi Ihara
  • Organizer: International Stroke Conference
  • Place of Presentation: Houston, USA
  • Year and Date: 2017-02-21
  • Int'l Joint Research / Invited
• [Presentation] cnm遺伝子陽性Streptococcus mutansと脳微小出血の分布との関連 (2017)
  • Author(s): 殿村 修一, 齊藤 聡, 峰松 一夫, 猪原 匡史
  • Organizer: 脳ドック学会
• [Presentation] Dental microbes and stroke/dementia. (2016)
  • Author(s): Masafumi Ihara
  • Organizer: Brain Ageing and Dementia in LIMICS 2016
  • Place of Presentation: Nairobi, Kenya
  • Year and Date: 2016-12-06
  • Int'l Joint Research / Invited
• [Presentation] 脳卒中の新たな危険因子－口腔内感染－． (2016)
  • Author(s): 猪原匡史
  • Organizer: 日本神経学会学術大会
  • Place of Presentation: 神戸
  • Year and Date: 2016-05-18
• [Remarks] 国立循環器病研究センター病院・脳神経内科
  • URL: http://www.ncvc.go.jp/hospital/section/scd/cerebrovascular/b-group.html
• [Remarks] 国立循環器病研究センター病院脳神経内科
  • URL: http://www.ncvc.go.jp/hospital/section/scd/cerebrovascular/b-group.html