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The role of nuclear-mitochondrial interaction in mouse embryo implantation

Research Project

Project/Area Number 16K14587
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Laboratory animal science
Research InstitutionHokkaido University

Principal Investigator

Kawahara Manabu  北海道大学, 農学研究院, 准教授 (70468700)

Co-Investigator(Kenkyū-buntansha) 小川 英彦  東京農業大学, 生命科学部, 教授 (20339089)
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsミトコンドリア / 初期胚発生 / 着床 / 栄養膜幹細胞 / マウス / ウシ / 卵母細胞 / ヘテロプラスミー / ミトコンドリアゲノム / 核ゲノム / 哺乳類 / 顕微操作
Outline of Final Research Achievements

Mitochondria are present in every eukaryotic cell, and have unique DNA different from nuclear DNA. Compared with nuclear DNA, it has a close structure between species. However, mitochondrial heteroplasmic embryo (mtB-M embryo) in which heterologous mitochondria derived from cattle has been mixed in mouse embryos shows implantation failure. In this study, we examined the ability of mtB-M embryo to form trophoblast stem cells (TS cells) and investigated the cause of mtB-M embryo implantation failure. Mouse oocytes were subjected to in vitro fertilization, and subsequently, mitochondrial regions derived from bovine pronuclear stage embryos were transplanted into pronuclear stage embryos by the cell fusion. In the establishment test of TS cells, colonies derived from the mtB-M embryos could not be maintained until passage 5. In summary, the function of trophoblast cells cannot be maintained in the mtB-M embryos, which is considered to be one of causes of implantation failure.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (18 results)

All 2018 2017 2016 Other

All Journal Article (9 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 9 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (8 results) (of which Int'l Joint Research: 2 results) Remarks (1 results)

  • [Journal Article] Identification and expression analysis of cDNA encoding insulin-like growth factor 2 in horses2018

    • Author(s)
      Kikuchi K, Sasaki K, Akizawa H, Tsukahara H, Bai H, Takahashi M, Nambo Y, Hata H, Kawahara M.
    • Journal Title

      Journal of Reproduction and Development

      Volume: 64 Issue: 1 Pages: 57-64

    • DOI

      10.1262/jrd.2017-124

    • NAID

      130006405024

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Evaluating the electrical impedance and mucus-related gene expression of uterine endometrial tissues in mares2018

    • Author(s)
      Kikuchi K, Kozai K, Hojo T, Sakatani M, Okuda K, Bai H, Kawahara M, Takahashi M.
    • Journal Title

      Journal of Reproduction and Development

      Volume: 64 Issue: 2 Pages: 193-197

    • DOI

      10.1262/jrd.2017-128

    • NAID

      130006699687

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Reciprocal regulation of TEAD4 and CCN2 for the trophectoderm development of the bovine blastocyst.2018

    • Author(s)
      Hiroki Akizawa, Ken Kobayashi , Hanako Bai, Masashi Takahashi, Shinjiro Kagawa, Hiroaki Nagatomo, Manabu Kawahara
    • Journal Title

      Reproduction

      Volume: 印刷中 Issue: 6 Pages: 563-571

    • DOI

      10.1530/rep-18-0043

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enhancement of sperm motility and viability by turmeric by-product dietary supplementation in roosters2017

    • Author(s)
      Yan W, Kanno C, Oshima E, Kuzuma Y, Kim SW, Bai H, Takahashi M, Yanagawa Y, Nagano M, Wakamatsu JI, Kawahara M.
    • Journal Title

      Animal reproduction science.

      Volume: 185 Pages: 195-204

    • DOI

      10.1016/j.anireprosci.2017.08.021

    • NAID

      120006523735

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Pyridoxine supplementation during oocyte maturation improves the development and quality of bovine preimplantation embryos.2017

    • Author(s)
      Aboelenain M, Balboula AZ, Kawahara M, El-Monem Montaser A, Zaabel SM, Kim SW, Nagano M, Takahashi M.
    • Journal Title

      Theriogenology

      Volume: 91 Pages: 127-133

    • DOI

      10.1016/j.theriogenology.2016.12.022

    • NAID

      120006370595

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Estrous cycle stage-dependent manner of type I interferon-stimulated genes induction in the bovine endometrium2017

    • Author(s)
      Shirozu T, Iwano H, Ogiso T, Suzuki T, Balboula AZ, Bai H, Kawahara M, Kimura K, Takahashi H, Rulan B, Kim SW, Yanagawa Y, Nagano M, Imakawa K, Takahashi M.
    • Journal Title

      Journal of Reproduction and Development

      Volume: 63 Issue: 3 Pages: 211-220

    • DOI

      10.1262/jrd.2016-176

    • NAID

      130007314602

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Requirement for nuclear autoantigenic sperm protein mRNA expression in bovine preimplantation development.2016

    • Author(s)
      Nagatomo H., Kohri N., Akizawa H., Hoshino Y., Yamauchi N., Kono T., Takahashi M., and Kawahara M.
    • Journal Title

      Animal Science Journal

      Volume: 87 Issue: 3 Pages: 457-461

    • DOI

      10.1111/asj.12538

    • NAID

      120005981356

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Conserved roles of fibroblast growth factor receptor 2 signaling in the regulation of inner cell mass development in bovine blastocysts.2016

    • Author(s)
      Akizawa H., Nagatomo H., Odagiri H., Kohri N., Yamauchi N., Yanagawa Y., Nagano M., Takahashi M., and Kawahara M.
    • Journal Title

      Molecular Reproduction and Development

      Volume: 83 Issue: 6 Pages: 516-525

    • DOI

      10.1002/mrd.22646

    • NAID

      120006219875

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] The Influence of Polyploidy and Genome Composition on Genomic Imprinting in Mice.2016

    • Author(s)
      Yamazaki W., Amano T., Bai H., Takahashi M., and Kawahara M.
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 291 Pages: 20924-20931

    • NAID

      120006666502

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] ウシ-マウス間ミトコンドリアヘテロプラスミー胚の栄養膜細胞形成能.2018

    • Author(s)
      詫間光, 小松雅也, 唄花子, 高橋昌志, 小川英彦, 川原学.
    • Organizer
      日本生殖発生医学会 (東京)
    • Related Report
      2017 Annual Research Report
  • [Presentation] Effects of downregulating connective tissue growth factor transcripts on preimplantation development of bovine embryos.2017

    • Author(s)
      Akizawa H., Sada A., Nagatomo H., Bai H., Takahashi M. and KawaharaM.
    • Organizer
      50th Annual Meeting of the Society for the Study of Reproduction, Washington D.C. (USA)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Intracellur localizations of CDX2, TEAD4, and YAP proteins during preimplantation development in cattle.2017

    • Author(s)
      Kohri N., Kagawa S., Bai H, Takahashi M. and Kawahara M.
    • Organizer
      50th Annual Meeting of the Society for the Study of Reproduction, Washington D.C. (USA)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Cathepsin B regulation rescues the perturbation of spindle assembly checkpoint function in vitrified mouse oocytes.2016

    • Author(s)
      Ahmed Z. Balboula, 唄花子, 川原学, 小谷友也, 高橋昌志.
    • Organizer
      日本繁殖生物学会
    • Place of Presentation
      相模原(麻布大学)
    • Year and Date
      2016-09-11
    • Related Report
      2016 Research-status Report
  • [Presentation] 発情期及び妊娠認識時のウシ子宮組織におけるオートファジー-カテプシン関連因子の発現動態.2016

    • Author(s)
      鈴木惇文, 白水貴大, 岩野弘暉, 小木曽貴季, 山内伸彦, 柳川洋二郎, 永野昌志, 唄花子, 川原学, 高橋昌志.
    • Organizer
      日本繁殖生物学会
    • Place of Presentation
      相模原(麻布大学)
    • Year and Date
      2016-09-11
    • Related Report
      2016 Research-status Report
  • [Presentation] サブユニット特異的なI型インターフェロン(IFN)受容体発現抑制によるIFN-τシグナル関連遺伝子発現への影響.2016

    • Author(s)
      白水貴大, 鈴木惇文, 岩野弘暉, 小木曽貴季, 金星佑, 唄花子, 川原学, 木村康二, 高橋昌志.
    • Organizer
      日本繁殖生物学会
    • Place of Presentation
      相模原(麻布大学)
    • Year and Date
      2016-09-11
    • Related Report
      2016 Research-status Report
  • [Presentation] マウス胎子の倍数性変化がインプリント遺伝子発現とDMRメチル化状態に及ぼす影響.2016

    • Author(s)
      山﨑渉, 天野朋子, 唄花子, 高橋昌志, 川原学.
    • Organizer
      日本繁殖生物学会
    • Place of Presentation
      相模原(麻布大学)
    • Year and Date
      2016-09-11
    • Related Report
      2016 Research-status Report
  • [Presentation] MII期核置換による再構築卵母細胞由来マウスの出生後の生存性.2016

    • Author(s)
      椎名浩己, 唄花子, 高橋昌志, 川原学.
    • Organizer
      日本卵子学会
    • Place of Presentation
      新潟(朱鷺メッセ)
    • Year and Date
      2016-05-14
    • Related Report
      2016 Research-status Report
  • [Remarks]

    • URL

      http://lab.agr.hokudai.ac.jp/anim/breed/index.html

    • Related Report
      2017 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2019-03-29  

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