Generation and Analysis of Antigen-specific T cell-deficient mice by CRISPR/Cas9.
| Project/Area Number |
16K14591
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Watarai Hiroshi 東京大学, 医科学研究所, 特任准教授 (70415339)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | ゲノム編集 / CRISPR/Cas9 / iNKT細胞 / 肥満 / CD1d / ゲノム |
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| Outline of Final Research Achievements |
Here we have successfully generated new Traj18－/－ mouse lines by using the Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/Cas9 technology. The CRISPR-Traj18－/－ mice lacked iNKT cells and harbored an undisturbed TCRα repertoire, fulfilling the criteria of iNKT cell-deficient mice.
Divergent findings for the metabolic role of iNKT cells have been reported in studies using the previously generated Traj18－/－ mouse strain. Therefore, we re-investigated the contribution of iNKT cells to the development of obesity induced by a high-fat diet (HFD) using our novel Traj18－/－ mouse strain on the B6 background.Among the experimental groups on HFD, CRISPR-Traj18－/－ (1-1L) mice gained less weight than WT B6 mice. The CRISPR-Traj18－/－ mouse strain on HFD also exhibited ameliorated metabolic phenotypes, which is consistent with a pathogenic role of iNKT cells in the development of obesity and insulin-resistance.
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Report
(3 results)
Research Products
(17 results)
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[Journal Article] Ultrafast confocal fluorescence microscopy beyond the fluorescence lifetime limit2018
Author(s)
Mikami H, Harmon J, Kobayashi H, Hamad S, Wang Y, Iwata O, Suzuki K, Ito T, Aisaka Y, Kutsuna N, Nagasawa K, Watarai H, Ozeki Y, Goda K.
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Journal Title
Optica
Volume: 5
Issue: 2
Pages: 117-126
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] IL-22BP dictates characteristics of Peyer's patch follicle-associated epithelium for antigen uptake.2017
Author(s)
Jinnohara T, Kanaya T, Hase K, Sakakibara S, Kato T, Tachibana N, Sasaki T, Hashimoto Y, Sato T, Watarai H, Kunisawa J, Shibata N, Williams I, Kiyono H, and Ohno H.
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Journal Title
J. Exp. Med.
Volume: 214
Issue: 6
Pages: 1607-1618
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] A Novel Mouse Model of iNKT Cell-deficiency Generated by CRISPR/Cas9 Reveals a Pathogenic Role of iNKT Cells in Metabolic Disease.2017
Author(s)
Ren Y, Sekine-Kondo E, Shibata R, Kato-Itoh M, Umino A, Yanagida A, Satoh M, Inoue K, Yamaguchi T, Mochida K, Nakae S, Van Kaer L, Iwabuchi K, Nakauchi H, Watarai H.
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Journal Title
Sci Rep
Volume: 7
Issue: 1
Pages: 12765-12765
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] High-speed stimulated Raman scattering microscopy for studying the metabolic diversity of motile Euglena gracilis.2017
Author(s)
Yuta Suzuki, Yoshifumi Wakisaka, Osamu Iwata, Ayaka Nakashima, Takuro Ito, Misa Hirose, Ryota Domon, Mai Sugawara, Norimichi Tsumura, Hiroshi Watarai, Tomoyoshi Shimobaba, Kengo Suzuki, Keisuke Goda, Yasuyuki Ozeki.
Organizer
SPIE BiOS
Related Report
Int'l Joint Research
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