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Elucidating the metabolic enzymes network in hypoxic cells using next-generation proteomics

Research Project

Project/Area Number 16K14612
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Tumor biology
Research InstitutionKyushu University

Principal Investigator

Oshikawa Kiyotaka  九州大学, 生体防御医学研究所, 学術研究員 (50380051)

Co-Investigator(Renkei-kenkyūsha) NAKAYAMA Keiichi  九州大学, 生体防御医学研究所, 主幹教授 (80291508)
Matsumoto Masaki  九州大学, 生体防御医学研究所, 准教授 (60380531)
Project Period (FY) 2016-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Keywordsプロテオミクス / 低酸素
Outline of Final Research Achievements

The metabolic network is specific changed in hypoxia conditions, but the precise expression pattern is poorly understood. By comparing hypoxic to normoxic metabolic enzyme levels in various cells, we established four cell lines (normal, cancer, senescent, and G0 cells) from normal diploid fibroblast (TIG-3). To quantify the metabolic enzyme levels (~1,000 enzymes) of hypoxic and normoxic conditions in four cell lines, we performed the in vitro proteome assisted MRM for protein absolute quantification (iMRM). We identified specific metabolic enzymes which were significantly changed in hypoxic conditions.

Report

(2 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • Research Products

    (3 results)

All 2017 2016

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (2 results)

  • [Journal Article] A large-scale targeted proteomics assay resource based on an in vitro human proteome.2017

    • Author(s)
      Matsumoto M, Matsuzaki F, Oshikawa K, Goshima N, Mori M, Kawamura Y, Ogawa K, Fukuda E, Nakatsumi H, Natsume T, Fukui K, Horimoto K, Nagashima T, Funayama R, Nakayama K, Nakayama KI.
    • Journal Title

      Nat Methods

      Volume: 14 Issue: 3 Pages: 251-258

    • DOI

      10.1038/nmeth.4116

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] SV40 small T antigenはoncogene-induced senescenceを効率的に回避するために必要である2016

    • Author(s)
      押川 清孝、松本 雅記、中山 敬一
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パフィシコ横浜・横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] 情報基盤多重モニタリング法(iMPAQT)を用いた細胞老化代謝ネットワークの解析2016

    • Author(s)
      押川 清孝、松本 雅記、中山 敬一
    • Organizer
      日本プロテオーム学会2016年大会
    • Place of Presentation
      北里大学・東京
    • Year and Date
      2016-07-28
    • Related Report
      2016 Annual Research Report

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Published: 2016-04-21   Modified: 2018-03-22  

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