Elucidating the metabolic enzymes network in hypoxic cells using next-generation proteomics
| Project/Area Number |
16K14612
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor biology
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAYAMA Keiichi 九州大学, 生体防御医学研究所, 主幹教授 (80291508)
Matsumoto Masaki 九州大学, 生体防御医学研究所, 准教授 (60380531)
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| Project Period (FY) |
2016-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Keywords | プロテオミクス / 低酸素 |
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| Outline of Final Research Achievements |
The metabolic network is specific changed in hypoxia conditions, but the precise expression pattern is poorly understood. By comparing hypoxic to normoxic metabolic enzyme levels in various cells, we established four cell lines (normal, cancer, senescent, and G0 cells) from normal diploid fibroblast (TIG-3). To quantify the metabolic enzyme levels (~1,000 enzymes) of hypoxic and normoxic conditions in four cell lines, we performed the in vitro proteome assisted MRM for protein absolute quantification (iMRM). We identified specific metabolic enzymes which were significantly changed in hypoxic conditions.
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Report
(2 results)
Research Products
(3 results)
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[Journal Article] A large-scale targeted proteomics assay resource based on an in vitro human proteome.2017
Author(s)
Matsumoto M, Matsuzaki F, Oshikawa K, Goshima N, Mori M, Kawamura Y, Ogawa K, Fukuda E, Nakatsumi H, Natsume T, Fukui K, Horimoto K, Nagashima T, Funayama R, Nakayama K, Nakayama KI.
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Journal Title
Nat Methods
Volume: 14
Issue: 3
Pages: 251-258
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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